PharmaOnco™ HEK293-Tg(Human TFRC) Stable Cell Line

Creative Biolabs offers HEK293-Tg(Human TFRC) stable cell line which the TFRC gene stably expressed in HEK293 cells.

HEK293-Tg(Human TFRC) stable cell line refers to a cell line derived from Human Embryonic Kidney (HEK) 293 cells that has been genetically engineered to stably express the human transferrin receptor protein (TFRC). TFRC is a cell surface receptor that binds to transferrin, a protein responsible for iron transport in the body. This stable cell line can be used in various research applications, including the study of iron metabolism, cellular uptake of iron, and the role of TFRC in various cellular processes. It can also be used as a tool for drug screening and development, particularly in the field of iron-related disorders and diseases.

Consistent gene expression;
Reliability and reproducibility;

Functional assay

TFRC

Human

HEK293

GenBank accession #NM_001128148

The TFRC gene encodes the transferrin receptor protein, which is responsible for the uptake of iron-bound transferrin into cells. This protein plays a crucial role in maintaining cellular iron homeostasis and is highly expressed in cells with high iron demands, such as rapidly dividing cells and erythroid precursors. Mutations in the TFRC gene can lead to various forms of iron-related disorders, such as hereditary hemochromatosis and iron-refractory iron deficiency anemia. Additionally, the TFRC gene has been implicated in cancer progression, as cancer cells often upregulate transferrin receptor expression to support their increased iron requirements.

1 x 10e6 cells/vial, Frozen

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.