PharmaOnco™ HEK293-Tg(Human TLR9)-NF-κB Luc Reporter Cell Line, Single Clone

CAT#: ITS-0822-HMM216
Product Type: Stable Cell Line
Short Description
Creative Biolabs offers HEK293-Tg(Human TLR9)-NF-κB Luc reporter cell line which the TLR9 gene stably expressed in NF-κB Luc reporter HEK293 cells.
Description
The HEK293-Tg(Human TLR9)-NF-κB Luc reporter cell line is a cell line derived from human embryonic kidney 293 cells that has been genetically engineered to overexpress human Toll-like receptor 9 (TLR9) and a luciferase reporter gene under the control of the NF-κB transcription factor promoter. This cell line is commonly used in research studies to investigate the activation of the NF-κB signaling pathway in response to TLR9 ligands, such as unmethylated CpG DNA, which are known to trigger immune responses.
Features
Consistent gene expression;
Reliability and reproducibility;
Applications
Functional assay
Protein Target
TLR9
Species
Human
Parental Cell Line
HEK293
Gene
GenBank Accession No. NM_017442
Background
The TLR9 gene encodes a protein called Toll-like receptor 9, which is a receptor involved in recognizing and responding to microbial DNA. TLR9 plays a critical role in the innate immune response by detecting pathogens and triggering an immune response to eliminate them. Mutations or dysregulation of the TLR9 gene have been associated with various autoimmune disorders and diseases, making it an important target for research in immunology and inflammation.
Unit Size
1 x 10e6 cells/vial, Frozen
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Shipping
Dry Ice
Storage
Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Quality Control
All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.
For Research Use Only | Not For Clinical Use
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