PharmaOnco™ HEK293T-Tg(Human GLP-2 Receptor) Division-Arrested Cell, Single Clone

Creative Biolabs offers HEK293T-Tg(Human GLP-2 Receptor) Division-Arrested Cell which GLP-2 receptor stably expressed in HEK293T cells.

HEK293T-Tg(Human GLP-2 Receptor) Division-Arrested Cell was engineered to express the receptor human GLP-2 (NM_004382.3). This cell line can be used to study GLP-2 receptor function, signaling pathways, and potential therapeutic interventions. Dividing-arrest cells are cells that are normally kept under specific culture conditions or treated with agents that prevent cell division from being held in a non-dividing state. This can be achieved through methods such as serum starvation, chemical inhibitors of cell cycle progression, or genetic modification.

Well-characterized stable cell lines;
for cell-based high-throughput screening;
Low-cost evaluation of stable cell lines or limited quantities of compounds.

GLP-2 receptor function, signaling pathways, and potential therapeutic interventions.

GPCR

GLP-2

Glucagon

Human

HEK293T

Expression vector containing full-length human CRHR1 cDNA (GenBank Accession Number NM_004382.3) with FLAG tag sequence at N-terminus

NM_004382.3

Glucagon-like peptide 2 receptor belongs to the glucagon-secretin receptor superfamily of GPCRs. The human GLP-2 receptor gene is localized on chromosome 17p13.3. The GLP-2 receptor exhibits ~50% amino acid identity with GLP-1 receptor. GLP-2 receptor binds to Glucagon-like peptide-2 (GLP-2) is a nutrient-responsive hormone and activates adenylate cyclase pathway and to a lesser extent activates MAP kinases. GLP-2 receptors are found in the central nervous system and gastrointestinal tract, with the highest expression levels in jejunum. The principal role of GLP-2 receptors appears to be the maintenance of the growth, nutrient absorption, cell proliferation, apoptosis, mucosal blood flow and suppressing gastric motility and secretion. The regenerative and cytoprotective properties of GLP-2 contribute to its therapeutic potential for the treatment of patients with intestinal disease. Recent studies have suggested that GLP-2 not only modulates intestinal stem cell behavior but may also promote carcinogenesis in models of sporadic colon cancer. Further consideration of the molecular cross-talk and downstream signaling pathways mediating the intestinotropic effects of GLP-2 is important. A detailed delineation of the signaling pathways activated by gut peptide GPCRs, as exemplified by GLP-1and GLP-2, may provide new therapeutic targets for the treatment of human disorders such as diabetes and intestinal disease, respectively

2 x 10e6 cells/vial, Frozen

DMEM, 10% FBS, 1 μg/mL puromycin

Cell freezing medium

>95%

>90%

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.