PharmaOnco™ HEK293T-Tg(Human NK1 Receptor) Division-Arrested Cell, Single Clone

Creative Biolabs offers HEK293T-Tg(Human NK1 Receptor) Division-Arrested Cell which NK1 receptor stably expressed in HEK293T cells.

HEK293T-Tg(Human NK1 Receptor) Division-Arrested Cell was engineered to express the receptor human NK1 (NM_001058.2). This cell line can be used to study NK1 receptor function, signaling pathways, and potential therapeutic interventions. Dividing-arrest cells are cells that are normally kept under specific culture conditions or treated with agents that prevent cell division from being held in a non-dividing state. This can be achieved through methods such as serum starvation, chemical inhibitors of cell cycle progression, or genetic modification.

Well-characterized stable cell lines;
for cell-based high-throughput screening;
Low-cost evaluation of stable cell lines or limited quantities of compounds.

NK1 receptor function, signaling pathways, and potential therapeutic interventions.

GPCR

NK1

Tachykinin

Human

HEK293T

Expression vector containing full-length human TACR1 cDNA (GenBank Accession Number NM_001058.2) with FLAG tag sequence at N-terminus

NM_001058.2

NK1 (or tachykinin receptor TACR1) is a selective receptor for substance P with relatively higher affinity for neurokinin A compared to neurokinin B (100- and 500-fold lower). Neurons in lamina I of the spinal dorsal horn express the NK1 receptor for substance P and mediate hyperalgesia, an enhanced sensitivity to painful stimuli. Although substance P does not mediate the signaling of acute pain or hyperalgesia, it is essential for the full development of stress-induced analgesia and for an aggressive response to territorial challenge. It is also suggested that substance P is important for orchestrating the response of the animal to major stressors such as pain, injury or invasion of territory. Modulation of substance P activity offers a radical new approach to the management of depression, anxiety and stress. The substance P receptor is highly expressed in areas of the brain that are implicated in these behaviors.

2 x 10e6 cells/vial, Frozen

DMEM, 10% FBS

Cell freezing medium

>95%

>90%

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.