PharmaOnco™ HEK293T-Tg(Human NTS2 Receptor) Division-Arrested Cell, Single Clone

Creative Biolabs offers HEK293T-Tg(Human NTS2 Receptor) Division-Arrested Cell which NTS2 receptor stably expressed in HEK293T cells.

HEK293T-Tg(Human NTS2 Receptor) Division-Arrested Cell was engineered to express the receptor human NTS2 (NM_012344.3). This cell line can be used to study NTS2 receptor function, signaling pathways, and potential therapeutic interventions. Dividing-arrest cells are cells that are normally kept under specific culture conditions or treated with agents that prevent cell division from being held in a non-dividing state. This can be achieved through methods such as serum starvation, chemical inhibitors of cell cycle progression, or genetic modification.

Well-characterized stable cell lines;
for cell-based high-throughput screening;
Low-cost evaluation of stable cell lines or limited quantities of compounds.

NTS2 receptor function, signaling pathways, and potential therapeutic interventions.

GPCR

NTS2

Neurotensin

Human

HEK293T

Expression vector containing full-length human NTSR2 cDNA (GenBank Accession Number NM_012344.3) with FLAG tag sequence at N-terminus

NM_012344.3

Neurotensin is a tridecapeptide that has been shown to act as a neurotransmitter/neuromodulator in the central nervous system and as a local hormone in the periphery. Human NTS2 is a 410 amino acid transmembrane protein in the neurotensin receptor family. It is primarily expressed in the central nervous system and in peripheral tissues. The neurotensin family plays an important role in a wide range of biological activities, such as hypotension, hyperglycemia, and regulation of vascular permeability. NTS2 has a low affinity for neurotensin, but SR 48692 has been proven to be a strong agonist in many recent studies. NTS2 may be an important target in the treatment of pain and stress-related disorders.

2 x 10e6 cells/vial, Frozen

Cell freezing medium

>95%

>90%

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.