PharmaOnco™ Human K562 Luc Reporter Tumor Cell Line

CAT#: IOC-ZP002
Product Type: Cell Line
Organism: Human
Short Description
Creative Biolabs' bioluminescent light-producing oncology cell lines are labeled with luciferase or dual labeled with luciferase and fluorescent reporters (GFP) for in vivo studies.
Applications
in vivo Imaging
Organism
Human
Strain Name
K562
Tissue
Bone Marrow
Disease
Chronic Myelogenous Leukemia
Optical Reporter
Luciferase
Oncology Cell Line
Blood
Quantity
1.0 Units
Cell Purity
>95%
Cell Viability
>90%
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Shipping
Dry Ice
Storage
Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Quality Control
All of our bioluminescent/reporter tumor cell lines meet the highest quality standards and have been confirmed to be pathogen-free.
COA

IOC-ZP002-1 Fig.1 Cell morphology of PharmaOnco™ Human K562 Luc Reporter Tumor Cell Line

Cell Morphology

Cell morphology under the bright field.

IOC-ZP002-2 Fig.2 Cell morphology and GFP observation of PharmaOnco™ Human K562 Luc Reporter Tumor Cell Line

Cell Morphology

Cell morphology and GFP observation under fluorescence microscopy.

IOC-ZP002-3 Fig.3 GFP expression in PharmaOnco™ Human K562 Luc Reporter Tumor Cell Line

GFP Expression Test

The GFP intensity was detected by FACS.

IOC-ZP002-5 Fig.5 Mycoplasma Testing of PharmaOnco™ Human K562 Luc Reporter Tumor Cell Line

Mycoplasma Testing

Mycoplasma contamination is detected by using a PCR Mycoplasma Test Kit. The kit utilizes the polymerase chain reaction (PCR), which was established as the method of choice for highest sensitivity in the detection of mycoplasma and acholeplasma contamination in cell cultures and other cell culture derived biologicals.
The results showed that the cell line was negative for Mycoplasma.
Lane M: Marker;
Lane 1-2: Cell culture medium from K562 LUC;
Lane 3: Negative control;
Lane 4: Positive control;

IOC-ZP002-4 Fig.4 Firefly luciferase in PharmaOnco™ Human K562 Luc Reporter Tumor Cell Line

Luciferase Testing

The luminescence intensity was detected by Bright-GloTM luciferase Assay system (Promega, Cat E2610)

For Research Use Only | Not For Clinical Use
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