PharmaOnco™ Hybridoma Growth Medium

Hybridoma growth medium with hypoxanthine and thymidine (serum-containing)

Creative Biolabs provide several clonal selection & culture media and related reagents to accelerate antibody development projects for clients.PharmaOnco™ Hybridoma Growth Medium is a serum-containing, nutritionally rich liquid medium optimized for hybridoma expansion after hypoxanthine, aminopterin, thymidine (HAT) selection. The medium contains hypoxanthine and thymidine (HT) and is used to wean hybridomas off aminopterin used during the selection process. This medium has been verified for use in mouse and rat hybridoma development and monoclonal antibody production and compatible for production, cloning, and expansion of hybridomas using lymphocytes from a variety of host animals including human, mouse, rat, and hamster.

lot-to-lot consistency;
the ease of downstream cell culture/purification of target proteins.

Cell Culture, Hybridoma Generation

Antibody Development, Cell Line Development, Drug Discovery and Toxicity Testing, Hybridoma Generation

500 mL

• DMEM
• Serum
• Hypoxanthine and thymidine (HT)
• Gentamicin
• 2-Mercaptoethanol
• Phenol red
• L-Glutamine and other supplements
• Other ingredients

Mouse

Store at +2°C to +8°C, protected from light;
Once opened store at 4°C and use within 6-8 weeks;

18 months from date of manufacture

Ambient