PharmaOnco™ Jurkat-NFAT Luc//eGFP Reporter Cell Line, Single Clone

Creative Biolabs offers Jurkat-NFAT Luc//eGFP reporter cell line which has been engineered to express both a luciferase reporter gene under the control of NFAT response elements, as well as eGFP.

The Jurkat-NFAT Luc//eGFP reporter cell line is a stable cell line derived from Jurkat T lymphocytes that has been engineered to express both a luciferase reporter gene under the control of NFAT response elements, as well as enhanced green fluorescent protein (eGFP). NFAT (Nuclear Factor of Activated T cells) is a key transcription factor that regulates immune response genes, and its activation can be monitored by measuring luciferase activity in response to various stimuli. The eGFP gene allows for tracking of the cells under a fluorescent microscope. This cell line is commonly used in research to study the signaling pathways involved in T cell activation and immune response.

Consistent gene expression;
Reliability and reproducibility;

Functional assay

Jurkat

NFAT Luc//eGFP Reporter is a tool used in research to study the activation of the NFAT signaling pathway, which plays a critical role in immune response, cell proliferation, and development. The reporter consists of a luciferase gene and an enhanced green fluorescent protein (eGFP) gene under the control of NFAT-responsive elements. When the NFAT signaling pathway is activated, NFAT translocates to the nucleus and induces the expression of the luciferase and eGFP genes, allowing for the detection and quantification of NFAT activation in cells. This reporter system is commonly used in cell culture studies and in vivo animal models to investigate the regulation of NFAT signaling in various biological processes.

1 x 10e6 cells/vial, Frozen

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.