PharmaOnco™ Jurkat-NFAT Luc//eGFP Reporter Cell Line, Single Clone (CAT#: ITS-0822-HMM183)

Creative Biolabs offers Jurkat-NFAT Luc//eGFP reporter cell line which has been engineered to express both a luciferase reporter gene under the control of NFAT response elements, as well as eGFP.

The Jurkat-NFAT Luc//eGFP reporter cell line is a stable cell line derived from Jurkat T lymphocytes that has been engineered to express both a luciferase reporter gene under the control of NFAT response elements, as well as enhanced green fluorescent protein (eGFP). NFAT (Nuclear Factor of Activated T cells) is a key transcription factor that regulates immune response genes, and its activation can be monitored by measuring luciferase activity in response to various stimuli. The eGFP gene allows for tracking of the cells under a fluorescent microscope. This cell line is commonly used in research to study the signaling pathways involved in T cell activation and immune response.

Consistent gene expression;
Reliability and reproducibility;

Functional assay

Jurkat

NFAT Luc//eGFP Reporter is a tool used in research to study the activation of the NFAT signaling pathway, which plays a critical role in immune response, cell proliferation, and development. The reporter consists of a luciferase gene and an enhanced green fluorescent protein (eGFP) gene under the control of NFAT-responsive elements. When the NFAT signaling pathway is activated, NFAT translocates to the nucleus and induces the expression of the luciferase and eGFP genes, allowing for the detection and quantification of NFAT activation in cells. This reporter system is commonly used in cell culture studies and in vivo animal models to investigate the regulation of NFAT signaling in various biological processes.