PharmaOnco™ Jurkat-NFAT Luc Reporter Cell Line, Single Clone

Creative Biolabs offers Jurkat-NFAT Luc reporter cell line which has been engineered to express a luciferase reporter gene under the control of the NFAT pathway.

The Jurkat-NFAT Luc reporter cell line is a genetically modified cell line derived from Jurkat cells, a human T lymphocyte cell line. These cells have been engineered to express a luciferase reporter gene under the control of the nuclear factor of activated T cells (NFAT) pathway. The NFAT pathway is a crucial signaling pathway in T cells that plays a role in their activation and differentiation. When the NFAT pathway is triggered, NFAT translocates to the nucleus and activates the transcription of target genes, including the luciferase gene in the Jurkat-NFAT Luc reporter cell line. This cell line is widely used in immunology and drug discovery research to study T cell signaling and screen for potential immunomodulatory compounds.

Consistent gene expression;
Reliability and reproducibility;

Functional assay

Jurkat

The NFAT Luc Reporter is a molecular reporter system used in cell biology to monitor the activity of the transcription factor NFAT (nuclear factor of activated T cells). NFAT is a key regulator of immune and inflammatory responses, as well as other cellular processes. The NFAT Luc Reporter consists of a gene encoding the luciferase enzyme under the control of NFAT-responsive elements. When NFAT is activated and translocates to the nucleus, it binds to these elements and induces the expression of luciferase. This results in the production of light (luminescence) that can be easily detected and quantified using a luminometer or other imaging systems.

1 x 10e6 cells/vial, Frozen

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.