PharmaOnco™ Jurkat-Tg(Human CD27)-NF-kB Reporter Cell Line, Single Clone

Creative Biolabs offers Jurkat-Tg(Human CD27)-NF-kB reporter cell line which the CD27 gene stably expressed in NF-κB Luc reporter Jurkat cells.

The Jurkat-Tg(Human CD27)-NF-kB reporter cell line is a cell line derived from Jurkat cells that have been genetically modified to express human CD27 and a reporter gene for NF-kB activity. CD27 is a cell surface molecule that is involved in T cell activation and differentiation, while NF-kB is a transcription factor that plays a key role in regulating immune and inflammatory responses. This cell line can be used in research to study the signaling pathways associated with CD27 activation and the downstream effects on NF-kB activity. It can also be used to screen for potential novel therapeutics that modulate CD27 signaling or NF-kB activation.

Consistent gene expression;
Reliability and reproducibility;

Functional assay

CD27

Human

Jurkat

Genbank Accession #BC012160

The CD27 gene, also known as TNFRSF7, encodes a protein that is a member of the tumor necrosis factor receptor superfamily. CD27 is primarily expressed on the surface of T cells and plays a role in T cell activation, survival, and differentiation. It is also involved in the immune response to pathogens and cancer cells. Mutations in the CD27 gene have been associated with immunodeficiency disorders and autoimmune diseases. Therapeutic targeting of CD27 is being explored for the treatment of cancer and inflammatory conditions.

1 x 10e6 cells/vial, Frozen

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.