PharmaOnco™ Jurkat-Tg(Human Foxp3)-Luc Reporter Cell Line, Single Clone

CAT#: ITS-0822-HMM156
Product Type: Stable Cell Line
Short Description
Creative Biolabs offers Jurkat-Tg(Human Foxp3)-Luc reporter cell line which the Foxp3 gene stably expressed in Luc reporter Jurkat cells.
Description
The Jurkat-Tg(Human Foxp3)-Luc reporter cell line is a type of Jurkat T cell line that has been genetically modified to express a luciferase reporter gene under the control of the human Foxp3 promoter. This allows researchers to easily monitor the expression of the Foxp3 gene, which is a master regulator of regulatory T cell function. These cells are commonly used in immunology research to study the role of regulatory T cells in immune responses, autoimmune diseases, and cancer.
Features
Consistent gene expression;
Reliability and reproducibility;
Applications
Functional assay
Protein Target
Foxp3
Species
Human
Parental Cell Line
Jurkat
Background
The Foxp3 gene is a gene that plays a critical role in the regulation of the immune system. It is primarily expressed in regulatory T cells (Tregs), which are a subset of T cells that help maintain immune tolerance and prevent autoimmune diseases. Mutations in the Foxp3 gene can lead to a severe immune disorder called immune dysregulation, polyendocrinopathy, enteropathy, X-linked (IPEX) syndrome. This syndrome causes a variety of autoimmune symptoms, including diabetes, thyroid disease, and inflammatory bowel disease.
Unit Size
1 x 10e6 cells/vial, Frozen
Sterility Testing
Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Identity Testing
Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.
Virological Safety Testing
A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Shipping
Dry Ice
Storage
Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.
Handling Notes
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.
Warnings
Avoid multiple freeze/thaw cycles
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Quality Control
All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.
For Research Use Only | Not For Clinical Use
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