PharmaOnco™ Jurkat-Tg(Human GITR)-NF-κB Reporter Cell Line, Single Clone

Creative Biolabs offers Jurkat-Tg(Human GITR)-NF-κB reporter cell line which the GITR gene stably expressed in NF-κB Luc reporter Jurkat cells.

The Jurkat-Tg(Human GITR)-NF-κB reporter cell line is a cell line derived from Jurkat cells that have been engineered to overexpress the human glucocorticoid-induced tumor necrosis factor receptor (GITR) gene and contain a NF-κB reporter gene. This cell line is used as a tool for studying the NF-κB signaling pathway in response to GITR activation. GITR is a member of the TNF receptor superfamily and is known to play a role in regulating immune responses, particularly T cell activation. Activation of GITR has been shown to enhance T cell proliferation and cytokine production, making it a potential target for immunotherapy.

Consistent gene expression;
Reliability and reproducibility;

Functional assay

GITR

Human

Jurkat

The GITR gene, also known as Glucocorticoid-Induced TNFR-Related Protein, encodes a protein that is a member of the tumor necrosis factor receptor superfamily. This protein is involved in the regulation of various immune responses, particularly in the activation of T cells. It has been studied for its potential role in cancer immunotherapy, as it has been shown to enhance the anti-tumor immune response when targeted with agonistic antibodies. Additionally, the GITR gene has also been implicated in the regulation of autoimmune diseases and inflammation.

1 x 10e6 cells/vial, Frozen

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.