PharmaOnco™ Jurkat-Tg(Human LAG3)-IL-2 Luc Reporter Cell Line, Single Clone

Creative Biolabs offers Jurkat-Tg(Human LAG3)-IL-2 Luc reporter cell line which the LAG3 gene stably expressed in IL-2 Luc reporter Jurkat cells.

The Jurkat-Tg(Human LAG3)-IL-2 Luc reporter cell line is a genetically modified cell line derived from the Jurkat cell line, a widely used human T cell lymphoma line. This specific cell line has been engineered to express a luciferase reporter gene under the control of the IL-2 promoter, allowing for the monitoring of IL-2 gene expression. Additionally, these cells also overexpress the human Lymphocyte-activation gene 3 (LAG3) protein, which is an immune checkpoint receptor that plays a role in regulating T cell function and immune responses.

Consistent gene expression;
Reliability and reproducibility;

Functional assay

LAG3

Human

Jurkat

GenBank Accession # NM_002286

The Lymphocyte-activation gene 3 (LAG3) is a protein that plays a role in regulating the immune response. It is a cell surface molecule that is expressed on activated T cells, regulatory T cells, and natural killer (NK) cells. LAG3 acts as an immune checkpoint molecule, similar to other checkpoint proteins like PD-1 and CTLA-4, and plays a role in regulating the activation and function of T cells. Binding of LAG3 to its ligands, such as MHC class II molecules, leads to inhibition of T cell proliferation and cytokine production, as well as promoting immune tolerance. Research has suggested that LAG3 may be a potential target for cancer immunotherapy, as blocking LAG3 can enhance anti-tumor immune responses. Studies are ongoing to investigate the potential of targeting LAG3 in cancer treatment.

1 x 10e6 cells/vial, Frozen

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.