PharmaOnco™ Raji-Tg(Human CD80//CD86) Stable Cell Line, Cell Pool/Single Clone

Creative Biolabs offers Raji-Tg(Human CD80//CD86) stable cell line which the CD80 and CD86 gene stably expressed in Raji cells.

Creative Biolabs offers the overexpression Raji cells that stably express immune checkpoints.

Consistent gene expression;
Reliability and reproducibility;

Functional assay

CD80 and CD86

Human

Raji

CD80, also known as B7-1, is a co-stimulatory molecule expressed on the surface of antigen-presenting cells (APCs), including dendritic cells, macrophages, and B cells. It plays a critical role in the activation of T cells by providing essential secondary signals necessary for T cell proliferation and survival. CD80 binds to the CD28 receptor on T cells, enhancing their activation when antigen is presented through the T cell receptor (TCR). Additionally, CD80 can interact with CTLA-4, leading to negative signaling that inhibits T cell activation. This dual role makes CD80 essential for regulating the immune response, promoting effective T cell activation while also maintaining tolerance to prevent autoimmune reactions.
CD86, also known as B7-2, is another co-stimulatory molecule found on antigen-presenting cells, such as dendritic cells, macrophages, and B cells. Like CD80, CD86 binds to CD28 on T cells to provide crucial signals that enhance T cell activation, proliferation, and cytokine production. CD86 can also interact with CTLA-4, but it offers a more transient signal compared to CD80. This interaction allows for fine-tuning of T cell responses, acting as a facilitator for activating T cells during the immune response while contributing to regulation and prevention of excessive activation. Together, CD80 and CD86 are fundamental for the initiation and regulation of adaptive immune responses.

1 vial

1 x 10e6 cells/vial, Frozen

Depends on the specific lot number

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only. Not for use in diagnostic procedures.

All of our cell lines meet the highest quality standards and have been confirmed to be pathogen-free.