IOCyto Detect™ CSF2 (GM-CSF) Rat ELISA Kit

CAT#: ITS-0622-CR8
Product Type: Oncology Kit
Target: GM-CSF
Short Description
CSF2 (GM-CSF) Rat ELISA Kit
Homogenous (no wash)
No
Interassay CV
12%
Intraassay CV
10%
Detector antibody conjugate
Biotin
Label or dye
HRP
Description
It is designed to detect rat CSF2 in rat serum, plasma, or cell culture medium. The assay can accurately measure both natural and recombinant rat CSF2.
Applications
Sandwich ELISA
Target
GM-CSF
Reactivity
Rat
Detection Method
Sandwich ELISA
Method Type
ELISA
Sample Type
Serum; plasma and cell culture supernatant
Specificity
Rat
Binding Specificity
Rat GM-CSF
Research Areas
Immuno-oncology
Size
96 Tests
Components
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Material not included
Distilled or deionized water
Microtiter Plate Reader software capable of measuring at 450 nm
Dishwasher - Automatic or Manual (Dispenser)
Calibrate adjustable precision pipettes and glass or plastic tubes to dilute solutions
Sensitivity
10 pg/mL
Sample Volume
50 µL to 100 µL
Assay Time
Less than 6 hours
Plate
96 well plate
Reagent Preparation
1. Collect samples in pyrogen/endotoxin-free tubes.
2. If the sample cannot be tested immediately after collection, freeze the sample. Freeze samples to avoid multiple freeze-thaw cycles. Thaw completely and mix well (do not vortex) prior to analysis.
3. Avoid using hemolytic or blood lipid serum. If a large amount of particulate matter is present in the sample, centrifuge or filter the sample prior to analysis.
Assay Procedure
1. Sample Incubation
2. Plate Washing
3. Biotinylated Antibody Reagent Incubation
4. Streptavidin-HRP Solution Preparation and Incubation
5. Substrate Incubation and Stop Step
6. Absorbance Measurement
Note: Evaluate the plate within 30-45 minutes of stopping the reaction.
Calculation of Results
1. Read the absorbance at 450nm. Read the plate within 30 minutes of adding the stop solution.
2. Generate a standard curve using curve fitting software.
3. Read the concentrations of unknown samples and controls from the standard curve. The sample dilution is corrected by an appropriate factor, resulting in a number of values (s) for the sample.
Assay Precision
12.35-3000 pg/mL
Format
Sandwich ELISA Kit
Precaution of Use
All products are supplied for research and laboratory use only.
Handling Advice
All blood components and biological materials should be treated with precautions as potentially hazardous. Strictly follow the management principles of the Centers for Disease Control and Prevention, Occupational Safety and Health Administration for handling and disposing of infectious agents.
Storage
The ELISA Kits are shipped at 2 to 8°C. Upon receipt, store the kits at 2 to 8°C.
Some components may need storage at -20°C.
Expiry Date
Stability If properly stored, all components are stable for up to 12 months. For expiry dates for the entire kit, see the kit label. The expiration date of each ingredient is shown on the bottle label. The expiration date of a kit ingredient is guaranteed only if the ingredient is properly stored and, in the event of repeated use of an component, the reagent will not be contaminated by the first treatment.
Note
Each production lot of this ELISA kit is quality tested to meet criteria such as sensitivity, specificity, precision and lot-to-lot consistency. See the manual for more information on validation.
Shipping
Wet or Dry Ice
Alternative Name
CSF2; GMCSF; colony stimulating factor 2; CSF
Background
It is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. This gene plays a role in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been detected in SARS-CoV-2 infected patients that develop acute respiratory distress syndrome. Mice deficient in this gene or its receptor develop pulmonary alveolar proteinosis.
For Research Use Only | Not For Clinical Use
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