Jurkat-Clone E6-1 In Vitro Nitric Oxide Synthases Assay (Oxidative Stress)

This assay is to provide Jurkat-Clone E6-1-based In Vitro Nitric Oxide Synthases Assay (Oxidative Stress) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

Jurkat-Clone E6-1

Human

Jurkat, Clone E6-1 is a clone of the Jurkat-FHCRC cell line, a derivative of the Jurkat cell line, which was established from the peripheral blood of a 14-year-old, male, acute T-cell leukemia patient. This cell line can be used in immune system disorder research and immunology and immuno-oncology research.

Acute Lymphoblastic Leukemia; ALL

Oncology

In Vitro Nitric Oxide Synthases Assay (Oxidative Stress)

Jurkat-Clone E6-1-cell based In Vitro Nitric Oxide Synthases Assay (Oxidative Stress)

Wide ranges of enzyme assays are also available to detect oxidative stress at the cellular level. Enzymes such as NO synthases and xanthine oxidase are known to generate ROS. Enzymes namely superoxide dismutase, catalase and thioredoxin reductase can prevent cells from oxidative damage. GST is another enzyme involved in oxidative stress that catalyzes the reduction of oxidized GSH to GSH.

Oxidative Stress Assays

Disturbance between the production of reactive oxygen species (ROS), free radicals and antioxidant mechanisms is defined as the oxidative stress, or more precisely, it is an imbalance between the oxidant and antioxidant state in cells. This imbalance can cause harmful effects to cells and biomolecules, which ultimately causes adverse effects in the whole organism. Oxidative imbalance can target important proteinsand lipids in cells, which can increase the risk of developing a cancer. On the other hand, increased ROS production in cancer cells by certain cancer drugs can also arrest cancer cell cycle and cause senescence and apoptosis through oxidative stress.

Enzyme Assay