Introduction

Proliferation assays are pivotal tools in the field of oncology research providing crucial insights into the growth dynamics and treatment responses of various cell lines. K562 cell line, derived from human chronic myelogenous leukemia, represents a valuable model for understanding leukemia proliferation and for testing potential therapeutic compounds. At Creative Biolabs, we specialize in leveraging the K562 cell line to provide high-quality, accurate proliferation assays that cater to diverse research needs.

K562-Based Proliferation Assay at Creative Biolabs

At Creative Biolabs, our K562-based proliferation assays are meticulously designed to meet the highest standards of scientific rigor. Our assays are designed to measure the inhibition of cell growth, quantify apoptotic responses, and elucidate signaling pathways involved in cell survival mechanisms. Utilizing a combination of high-throughput screening techniques and advanced analytical tools, we ensure precise and reliable results.

Proliferation Assay Types Provided

Creative Biolabs offers an array of proliferation assays tailored to meet specific research needs:

• MTT Assay: A cornerstone in cell proliferation analysis, the MTT assay evaluates mitochondrial activity as an indicator of cell viability and growth.
• BrdU Assay: The BrdU assay is an effective technique to study cell cycle dynamics and proliferation, employing the thymidine analog, Bromo-deoxyuridine (BrdU).
• Cell Viability Assays: Ranging from simple trypan blue exclusion to sophisticated luminescence-based assays, these methods assess cell viability by quantifying metabolic activity.
• Flow Cytometry-based Proliferation Test: An advanced technique to analyze cell populations based on specific markers, flow cytometry can identify circulating tumor cells and assess the efficacy of therapeutic agents.
• CFSE-Based Assays: Using CFSE dye, this assay evaluates cell proliferation and motility, offering insights into cell division and migration dynamics.
• Luminescent ATP Assay: The luminescence-based ATP assay measures the ATP content as a proxy for metabolically active cells, quantifying cell proliferation and viability.

Hot Luminescence-based K562 Proliferation Testing

Among these experiments, the luminescence-based assay is usually the most popular type of experiment for customers. The luminescence-based K562 proliferation assay measures cell viability by quantifying ATP levels, reflecting the presence of metabolically active cells. The convenience and high reproducibility of the assay make it more suitable for high-throughput screening.

Workflow

Scientific Backing

The use of K562 cells in proliferation studies is backed by extensive research demonstrating their utility in understanding leukemia biology and evaluating therapeutic compounds.

Frequently Asked Questions

Q1: How do you ensure the accuracy of your proliferation assays?

A1: Accuracy is ensured through stringent quality control measures, including the use of standardized protocols, calibration of equipment, and validation of assay results against known standards.

Q2: Can the proliferation assays be customized to specific research needs?

A2: Yes, we offer customization options to tailor the assays according to specific research requirements, including varying test concentrations and assay conditions.

Creative Biolabs is committed to delivering high-quality K562-based proliferation assays tailored to meet the diverse needs of our clients in the field of oncology research. Our expertise and commitment to excellence ensure that we continue to be a preferred partner in advancing scientific discoveries.

Reference

1. Wei, Zhaohui, et al. "Bone marrow mesenchymal stem cells from leukemia patients inhibit growth and apoptosis in serum-deprived K562 cells." Journal of Experimental & Clinical Cancer Research 28 (2009): 1-7. Distributed under Open Access License CC BY 4.0, without modification.

For Research Use Only | Not For Clinical Use