K562 In Vitro Calcein AM Cytotoxic Assay (Cell Viability)

CAT#: ITS-1022-YF112
Target Cell Organism: Human
Target Cell Alternative Name: K-562
Target Cell Name: K562
Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide K562-based In Vitro Calcein AM Cytotoxic Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
K562
Target Cell Organism
Human
Target Cell Background
K562 cells are of the erythroleukemia type, and the cell line is derived from a 53-year-old female chronic myelogenous leukemia patient in blast crisis. The cells are non-adherent and rounded, are positive for the bcr:abl fusion gene, and bear some proteomic resemblance to both undifferentiated granulocytes and erythrocytes.
Target Cell Alternative Name
K-562
Related Diseases
Chronic Myeloid Leukemia; CML
Research Area
Oncology
Assay Name
In Vitro Calcein AM Cytotoxic Assay (Cell Viability)
Short Description
K562-cell based In Vitro Calcein AM Cytotoxic Assay (Cell Viability)
Assay Description
Calcein AM is another non-fluorescent cell permeable dye, which is converted to green-fluorescent calcein by viable intracellular esterases. Calcein violet AM, calcein red-orange, Oregon green 488 and 5-CFDA-AM are other examples of dyes used to monitor cell viability based on esterase activity in viable cells.
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Viability Assay
Assay MOA
Cell-based Viability Assays Based on Cellular Enzymes and Proteins
For Research Use Only | Not For Clinical Use
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