Kasumi-3 In Vitro Calcein AM Cytotoxic Assay (Cell Viability)

CAT#: ITS-1122-YF3997
Target Cell Organism: Human
Target Cell Name: Kasumi-3
Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide Kasumi-3-based In Vitro Calcein AM Cytotoxic Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
Kasumi-3
Target Cell Organism
Human
Target Cell Background
The cell line was established from the blast cells of a myeloperoxidase-negative acute leukemia patient.
Treatment with either interleukin (IL)-2, IL-3, IL-4, granulocyte-macrophage colony-stimulating factor (GMCSF) or stem cell factor induced the proliferation, characteristic of undifferentiated leukemia.
Related Diseases
Acute Myeloblastic Leukemia; AML
Research Area
Oncology
Assay Name
In Vitro Calcein AM Cytotoxic Assay (Cell Viability)
Short Description
Kasumi-3-cell based In Vitro Calcein AM Cytotoxic Assay (Cell Viability)
Assay Description
Calcein AM is another non-fluorescent cell permeable dye, which is converted to green-fluorescent calcein by viable intracellular esterases. Calcein violet AM, calcein red-orange, Oregon green 488 and 5-CFDA-AM are other examples of dyes used to monitor cell viability based on esterase activity in viable cells.
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Viability Assay
Assay MOA
Cell-based Viability Assays Based on Cellular Enzymes and Proteins
For Research Use Only | Not For Clinical Use
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