L1210 In Vitro XTT Assay (Cell Viability)

This assay is to provide L1210-based In Vitro XTT Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

L1210

Mouse

L1210 is a cell line exhibiting lymphoblast morphology that was isolated from the skin of an eight-month-old, female mouse with lymphocytic leukemia, developed after skin paintings with 0.2% methylcholanthrene in ether. This cell line was deposited by EM Jensen and can be used in immune system disorder research.

Lymphocytic Leukemia

Oncology

In Vitro XTT Assay (Cell Viability)

L1210-cell based In Vitro XTT Assay (Cell Viability)

Apart from the MTT tetrazolium, other tetrazolium compounds such as XTT (2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) can also gener ate formazan upon reduction by oxidoreductase or dehydrogenase. Unlike the normal MTT assay, assays with these regents (MTS, XTT and WST) do not require formazan solubiliza tion step as solubilization occurs in the cell medium itself. Therefore, XTT, WST and MTS reagents can be used to assay viability of cells, which do not require a surface to attach.

XTT (2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) Assay

Cell Viability/Cytotoxicity and Antiproliferative Assays

Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.

Cell Viability Assay

Cell-based Viability Assays Based on Cellular Enzymes and Proteins