LC-MS based Bioanalysis Service
Creative Biolabs provides a comprehensive suite of LC-MS/MS bioanalytical services designed to support every stage of the drug discovery lifecycle. We provide end-to-end support, including de novo method development, high-throughput sample processing, and sophisticated data interpretation. By partnering with us, our clients gain access to cutting-edge mass spectrometry platforms and specialized sample preparation techniques that overcome complex matrix interference. The result is a significant gain in data reliability, shortened project timelines, and a deeper understanding of molecular behavior in biological systems.
LC-MS-based Bioanalysis Accelerating Drug Discovery and Development
Liquid chromatography-mass spectrometry (LC-MS) has emerged as a foundational tool in drug discovery, facilitating accurate and sensitive analysis of small molecules, peptides, proteins, and biomarkers. The comprehensive bioanalysis offered by LC-MS techniques is instrumental in understanding key parameters such as pharmacokinetics (PK), toxicokinetics (TK), bioavailability (BA), and exposure-response relationships, which play a key role in accelerating the drug development process and ensuring the safety and efficacy of drug products.
Prospective collaborators are invited to explore the potential for scientific partnership and may request a formal consultation to discuss specific research requirements.
Fig.1 Workflow of immunocapture-LC-MS/MS assays. 1
What We Can Offer
Equipped with advanced LC-MS technologies, Creative Biolabs offers tailored solutions for small molecules, peptides, and proteins using liquid chromatography-mass spectrometry (LC/MS). Our team of highly experienced scientists understands the unique requirements of each study and offers customized solutions tailored to clients' specific needs. From method development to sample analysis, our bioanalytical services are designed to support diverse studies, ranging from discovery through pre-clinical trials.
Expertise in Method Development and Validation
With experienced staff well-versed in the development and validation of small and mid-size peptides, fusion proteins, monoclonal antibodies, and biotherapeutics, we ensure high-quality and accurate results.
Effective Sample Processing and Analysis
We specialize in 96-well sample processing and analysis, and our bioanalytical methods cover a diverse range of compounds, including cardiovascular devices, free fatty acids, amino acids, and more.
Regulatory Compliance and GLP Standards
Our commitment to adhering to Good Laboratory Practices (GLP) ensures that all our bioanalytical methods meet the regulatory standards set by the OECD, and MHLW.
Rapid Turnaround Time and Sponsor Engagement
We collaborate closely with sponsors to ensure open lines of communication, transparency, and efficiency in our services.
Our Capabilities
| • Utilizing liquid/liquid, solid-phase extraction (SPE), and protein precipitation techniques in both standard and 96-well formats for comprehensive sample preparation. | • Implementing high-throughput automated liquid handling systems to efficiently accommodate studies of varying volumes. | • Offering in-depth analysis of peptides, pegylated drugs, and related metabolites to meet diverse research needs. | |
Application Area of LC-MS-based Bioanalysis Service
At Creative Biolabs, we leverage LC-MS for a diverse range of applications, including the analysis of oncology drugs, DNA adducts, free fatty acids, amino acids, and cardiovascular devices. Our expertise extends to developing and validating LC-MS/MS methods for a wide array of analytes across different matrices, highlighting the adaptability and robustness of our bioanalytical services.
How Creative Biolabs' service Can Assist Your Projects
Highlights
Nano-restricted Proteolytic Scission
We utilize orientation-limited enzymatic access to target discrete molecular domains. By liberating signature peptides, this methodology effectively minimizes stochastic interference from non-target scaffolds, yielding analytical clarity that surpasses conventional digestion paradigms.
Thresholds of Detection Sensitivity
Our workflows achieve exceptional quantitation limits within heterogeneous matrices, facilitating longitudinal monitoring of low-abundance biotherapeutics. Such precision is vital during early discovery to ensure subtle pharmacokinetic fluctuations are captured with high fidelity.
Consistency in Analytical Operations
We maintain a distinguished record of navigating technical complexities within international research collaborations. Our framework prioritizes operational rigor, ensuring that project deliverables remain academically robust, scientifically defensible, and meticulously scrutinized for accuracy.
High-Resolution Instrumentation Suites
Collaborators secure prioritized access to advanced platforms, including orbital-trap mass spectrometry and specialized chromatography. These tools offer the mass accuracy necessary to resolve structural challenges posed by diverse, modern drug modalities.
Prospective collaborators are invited to leverage the strategic advantages offered by Creative Biolabs. For a comprehensive project evaluation, please request a formal quotation.
Customer Reviews
-
Optimization of Ocular Pharmacokinetic Modeling
The integration of Creative Biolabs' specialized bioanalysis has refined the scientific understanding of vitreal distribution profiles. The deployment of a micro-flow LC configuration provided the necessary sensitivity for low-volume specimens derived from research models while maintaining robust chromatographic performance throughout the study. – M**l K***n. -
Analytical Integrity and Research Efficacy
Engagement with Creative Biolabs' bioanalytical services has facilitated the internal review processes. The resultant data packages demonstrated exceptional clarity, complemented by a proactive technical team that effectively addressed the multifaceted analytical requirements of a multi-site research initiative. – E**a R***z.
FAQs
How do you handle ion suppression in complex biological matrices?
We prioritize the "Three Goals" of sample prep: removing endogenous phospholipids, precipitating proteins, and concentrating the analyte. We often utilize supported liquid extraction (SLE) to ensure the cleanest possible extracts.
Can your LC-MS service replace traditional ELISA for my large molecule?
Yes, in many cases, LC-MS offers better selectivity, especially for distinguishing between parent drugs and metabolites. We can discuss a pilot study to transition your program.
Related Services
Immunoassay Service
Comprehensive detection services utilizing ELISA, RIA, and Multiplex platforms to quantify cytokines, biomarkers, and therapeutic proteins with high analytical sensitivity.
Learn More →In Vitro ADME Screening Service
High-throughput evaluation of metabolic stability, permeability, and drug-drug interaction potential utilizing cellular and microsomal models to characterize compound disposition and safety.
Learn More →How to Contact Us
With a focus on precision, efficiency, and regulatory compliance, our bioanalytical solutions are tailored to meet the diverse needs of pharmaceutical research. If you have any questions about the LC-MS-based bioanalysis services, please feel free to communicate with us.
Reference
- Chen, Lin-Zhi et al. "A Comprehensive Immunocapture-LC-MS/MS Bioanalytical Approach in Support of a Biotherapeutic Ocular PK Study." Pharmaceuticals (Basel, Switzerland) vol. 17,2 193. 31 Jan. 2024. Distributed under an Open Access license CC BY 4.0, without modification. https://doi.org/10.3390/ph17020193
Download our brochure