Measures glycerol release from adipocytes (lipolysis).
Brand
LipidFlux
Development Stage
Research-Grade Lipid Metabolism Assay Development
Description
The LipidFlux™ Adipolysis Assay Kit measures glycerol release to screen for compounds that stimulate or inhibit fat breakdown in adipocytes. This assay validates lipolytic agents and regulators of lipid mobilization, supporting the development of metabolic syndrome treatments. It provides a functional readout of adipocyte metabolism, essential for characterizing the mechanism of action of anti-diabetic drugs.
Features
Measures glycerol release; Screen for compounds that stimulate or inhibit fat breakdown in adipocytes.
Process Relevance
Lipid Mobilization Research
Application Stage
Lead Optimization
Applications
Lipid metabolism and fat cell regulation studies.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570 nm, or Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Cell culture
Research Areas
Obesity; Metabolic Disease
Size
200 tests
Data Output Type
Quantitative (Concentration)
Assay Time
Results in 20 min
LOD
0.92 µg/mL
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Lipolysis is the breakdown of triglycerides into glycerol and fatty acids. Stimulating lipolysis is a potential therapeutic strategy for obesity; this assay screens for compounds promoting fat breakdown.