LipidFlux™ Biotin Assay Kit

CAT#: ITS-1125-YL8
Product Type: Assay Kit
Species: All Species
Brand: LipidFlux
Development Stage: Research-Grade Lipid Metabolism Assay Development
Short Description
Quantifies biotin levels using a colorimetric method.
Brand
LipidFlux
Development Stage
Research-Grade Lipid Metabolism Assay Development
Description
This LipidFlux™ Biotin Assay Kit provides a sensitive colorimetric method for quantifying biotin levels in biological fluids and experimental samples. Utilizing a competitive binding mechanism with a specialized dye-avidin complex, the system measures the displacement of the dye to determine biotin concentrations. This reliable tool enables researchers to monitor biotin metabolism and protein labeling efficiency, supporting broad applications in cell signaling and metabolic research studies.
Features
Utilizes competitive binding technology to deliver rapid, cost-effective biotin quantification, facilitating nutritional research and biological sample analysis with high specificity and sensitivity.
Process Relevance
Quantifying biotinylation levels to verify protein labeling efficiency and support downstream conjugation processes.
Application Stage
Bioconjugation monitoring and quality control in protein labeling.
Applications
Determine biotin concentration and protein labeling efficiency in purified samples for optimized streptavidin-based detection and purification workflows.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 500 nm
Sample Type
Biological samples, food/feed extracts, cell culture media.
Research Areas
Proteomics; Molecular Biology; Biotechnology
Size
100 tests
Data Output Type
Quantitative concentration measurement
Assay Time
20 min
LOD
LOD: 8 µM
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
This water-soluble B-vitamin acts as a vital cofactor for carboxylase enzymes involved in fatty acid synthesis, gluconeogenesis, and amino acid metabolism. Its high affinity for specific proteins makes it a popular tool for labeling and detecting biological molecules in research.
Pathways
Biotin acts as a vital cofactor for several carboxylases in the fatty acid synthesis, gluconeogenesis, and amino acid catabolism pathways. It is essential for the upstream regulation of carbon dioxide transfer in metabolic carboxylation reactions.
For Research Use Only | Not For Clinical Use
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