LipidFlux™ Coenzyme A Assay Kit

CAT#: ITS-1125-HM108
Product Type: Assay Kit
Species: All Species
Short Description
Quantifies free Coenzyme A levels.
Brand
LipidFlux
Development Stage
Research-Grade Lipid Metabolism Assay Development
Description
The LipidFlux™ Coenzyme A Assay Kit utilizes enzyme cycling to increase sensitivity, critical for assessing metabolic flux and free acyl-CoA pools. This assay validates the availability of this essential cofactor in fatty acid and energy metabolism. It supports research into metabolic engineering and drug discovery, helping to understand the regulation of acetyl-CoA dependent pathways.
Features
Enzyme cycling increases sensitivity; Critical for assessing metabolic flux and acyl-CoA pools.
Process Relevance
Central Carbon Metabolism
Application Stage
Basic Research
Applications
Fatty acid metabolism and TCA cycle research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570 nm, or Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Metabolic Disease; Bioenergetics
Size
100 tests
Data Output Type
Quantitative (Concentration)
Assay Time
Results in 60 min
LOD
3 µM
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Coenzyme A (CoA) is a universal cofactor transporting acyl groups in metabolic pathways. Regulating free CoA levels is essential for maintaining metabolic flux in fatty acid oxidation and the TCA cycle.
For Research Use Only | Not For Clinical Use
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