Short Description
Quantifies free Coenzyme A levels.
Development Stage
Research-Grade Lipid Metabolism Assay Development
Description
The LipidFlux™ Coenzyme A Assay Kit utilizes enzyme cycling to increase sensitivity, critical for assessing metabolic flux and free acyl-CoA pools. This assay validates the availability of this essential cofactor in fatty acid and energy metabolism. It supports research into metabolic engineering and drug discovery, helping to understand the regulation of acetyl-CoA dependent pathways.
Features
Enzyme cycling increases sensitivity; Critical for assessing metabolic flux and acyl-CoA pools.
Process Relevance
Central Carbon Metabolism
Application Stage
Basic Research
Applications
Fatty acid metabolism and TCA cycle research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570 nm, or Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Metabolic Disease; Bioenergetics
Datasheet