LipidFlux™ Sphingomyelin Assay Kit

CAT#: ITS-1125-HM118
Product Type: Assay Kit
Species: All Species
Brand: LipidFlux
Development Stage: Research-Grade Lipid Metabolism Assay Development
Short Description
Quantifies sphingomyelin in lipid extracts.
Brand
LipidFlux
Development Stage
Research-Grade Lipid Metabolism Assay Development
Description
The LipidFlux™ Sphingomyelin Assay Kit uses enzymatic hydrolysis and fluorometric detection to validate drugs targeting sphingolipid signaling in apoptosis. This assay measures sphingomyelin levels in cell membranes and serum, supporting atherosclerosis research. It enables the investigation of lipid raft composition and the role of sphingolipids in signal transduction.
Features
Enzymatic hydrolysis and fluorometric detection; Validates drugs targeting sphingolipid signaling in apoptosis.
Process Relevance
Lipid Signaling Research
Application Stage
Discovery
Applications
Cell signaling and atherosclerosis research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 570 nm or Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Serum, plasma, and other biological samples
Research Areas
Cardiovascular Disease; Cell Signaling
Size
100 tests
Data Output Type
Quantitative (Concentration)
Assay Time
Results in 30 min
LOD
OD 1 mg/dL; FL 0.07 mg/dL
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Sphingomyelin is the dominant sphingolipid in cell membranes. Its metabolism generates bioactive ceramides involved in apoptosis and cell signaling; levels correlate with atherosclerosis risk.
For Research Use Only | Not For Clinical Use
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