LS 174T In Vitro PI Assay (Cell Viability)

CAT#: ITS-1122-YF332
Target Cell Organism: Human
Target Cell Name: LS 174T
Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide LS 174T-based In Vitro PI Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
LS 174T
Target Cell Organism
Human
Target Cell Background
LS 174T is a cell exhibiting epithelial morphology that was isolated from the colon of a White, 58-year-old, female adenocarcinoma patient with colorectal cancer. This cell line was deposited by Northwestern University and can be used in cancer research.
Related Diseases
Colorectal Adenocarcinoma; Colon Cancer
Research Area
Oncology
Assay Name
In Vitro PI Assay (Cell Viability)
Short Description
LS 174T-cell based In Vitro PI Assay (Cell Viability)
Assay Description
Propidium iodide (PI) is another dye that can be used to distinguish viable and non-viable cells. PI can only bind with DNA of cells with damaged cell membranes and it cannot enter viable cells. Uptake of PI into cells can be monitored by flow cytometry (FCM) or fluorescence microscopy.
Assay Alternative Names
Propidium Iodide Assay
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Viability Assay
Assay MOA
Dye Exclusion Assays
For Research Use Only | Not For Clinical Use
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