MB49 In Vitro (3)H-thymidine Incorporation Assay (Cell Proliferation)

This assay is to provide MB49-based In Vitro (3)H-thymidine Incorporation Assay (Cell Proliferation) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

MB49

Mouse

MB49 cells are derived from C57BL/Icrf-a' mouse bladder epithelial cells that were transformed by a single 24-hour treatment with the chemical carcinogen 7, 12-dimethylbenz[a]anthracene (DMBA) on the second day of a long term primary culture. Transformed cells transplanted into syngeneic mice were shown to generate carcinomas. While of male origin, karyotype analyses indicate the loss of the Y chromosome in 100% of the cells analyzed. This abnormality is a frequent early event in human bladder cancer.

Bladder Cancer

Oncology

In Vitro (3)H-thymidine Incorporation Assay (Cell Proliferation)

MB49-cell based In Vitro (3)H-thymidine Incorporation Assay (Cell Proliferation)

The 3H-thymidine incorporation assay is one such assay that can detect newly synthesized DNA in cells. Cultured cells are mixed with 3H-thymidine (radioactive) and kept for a desired incubation period. Newly replicated DNA strands can incorporate 3H-thymidine during mitosis. A scintillation counter is necessary to detect the output. Direct measurement of proliferating cells is the main advantage of this assay. However, use of harmful radioactive substances and need of special equipment are considered as main disadvantages.

Cell Viability/Cytotoxicity and Antiproliferative Assays

Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.

Cell Proliferation Assay

Cell-based Assays Based on DNA Synthesis