MB49 In Vitro Boyden Chamber Assay (Angiogenesis)

This assay is to provide MB49-based In Vitro Boyden Chamber Assay (Angiogenesis) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

MB49

Mouse

MB49 cells are derived from C57BL/Icrf-a' mouse bladder epithelial cells that were transformed by a single 24-hour treatment with the chemical carcinogen 7, 12-dimethylbenz[a]anthracene (DMBA) on the second day of a long term primary culture. Transformed cells transplanted into syngeneic mice were shown to generate carcinomas. While of male origin, karyotype analyses indicate the loss of the Y chromosome in 100% of the cells analyzed. This abnormality is a frequent early event in human bladder cancer.

Bladder Cancer

Oncology

In Vitro Boyden Chamber Assay (Angiogenesis)

MB49-cell based In Vitro Boyden Chamber Assay (Angiogenesis)

The Boyden chamber assay has been used to study angiogenesis by assessing endothelial cell migration.

Angiogenesis Monitoring Assays

Local and regional metastasis is a life-threatening condition in cancer, which is hard to control with the available cancer treatment options. In metastasis, cancer cells move away from primary tumors and travel through the blood and lymphatic system and form new tumors on other parts. A vascular
network growth is important for cancer metastasis. Angiogenesis is a process that is known as the formation of new blood vessels with the help of existing blood vessels and angiogenesis has been reported to play a major role in cancer growth and metastasis. Tumors can send chemical signals to nearby normal tissues to stimulate angiogenesis. New blood vessels can feed growing tumors with nutrients and oxygen, allowing cancer cells to spread (metastasis).