MB49 In Vitro Light Microscopy-based Apoptosis Assay

This assay is to provide MB49-based In Vitro Light Microscopy-based Apoptosis Assay to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

MB49

Mouse

MB49 cells are derived from C57BL/Icrf-a' mouse bladder epithelial cells that were transformed by a single 24-hour treatment with the chemical carcinogen 7, 12-dimethylbenz[a]anthracene (DMBA) on the second day of a long term primary culture. Transformed cells transplanted into syngeneic mice were shown to generate carcinomas. While of male origin, karyotype analyses indicate the loss of the Y chromosome in 100% of the cells analyzed. This abnormality is a frequent early event in human bladder cancer.

Bladder Cancer

Oncology

In Vitro Light Microscopy-based Apoptosis Assay

MB49-cell based In Vitro Light Microscopy-based Apoptosis Assay

For apoptosis-related morphological observations, light microscopy has widely been used. During apoptosis, changes in cell shape, volume, cell shrinkage and formation of apoptotic bodies can be clearly observed using light microscopy. Stains such as hematoxylin and eosin can be used to stain cancer cells before morphological observations under light microscopy. Although the light microscopy technique is an easy and inexpensive method, low sensitivity is the main disadvantage of this technique.

Detection of Apoptosis Assays

Apoptosis (programmed cell death) plays a vital role in embryonic development, homeostasis, functioning of immune system and wound repair. The ability to evade induction of apoptosis has been used by cancer cells to survive against host defense mechanisms. The molecular mechanisms involved in cancer cell apoptosis have been well documented and it involves certain biochemical events such as DNA fragmentation, chromatin condensation, cell organelle degradation and protein cleavage, etc. The extrinsic and intrinsic (mitochondrial) pathways are the two major pathways involved in apoptosis. With the available techniques and assays, a number of apoptosis inducing agents (natural compounds, synthetic compounds, nano-formulations, peptides and enzymes) in many cancer cells have been identified. Selection of an assay for apoptosis detection is based on factors such as apoptotic pathway, nature of drug, cell type being used and the method of analysis.

Microscopic Techniques to Detect Apoptosis Assays

Light Microscopy Assay