MBT-2 In Vitro Mitotic Index Assay (Cell Proliferation)

This assay is to provide MBT-2-based In Vitro Mitotic Index Assay (Cell Proliferation) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

MBT-2

Mouse

MBT-2 cells are murine bladder cancer cells with epithelial characteristics. Their formation is induced by the administration of FANFT (N-[4-(5-nitro-2-furyl)-2-thiazolyl] formamide) to C3H/He mice.

Bladder Cancer

Oncology

In Vitro Mitotic Index Assay (Cell Proliferation)

MBT-2-cell based In Vitro Mitotic Index Assay (Cell Proliferation)

Mitotic index assay is one of the old assays used to determine the number of cells that are undergoing mitosis in a specified cell population using monoclonal antibodies specific for phosphorylated histone H3 (ser28). Phosphorylated histone H3 (ser28) is considered as a main marker for mitosis and this specific phosphorylation can be utilized to calculate the mitotic index in a specified cell population.

Cell Viability/Cytotoxicity and Antiproliferative Assays

Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.

Cell Proliferation Assay

Cell-based Assays Based on DNA Synthesis