MetaVital™ Rat Glutathione S Transferase Alpha 1 (GSTa1) ELISA Kit

CAT#: ITS-0326-WB33
Product Type: ELISA Kit
Species: Rat
Target: Rat Glutathione S Transferase Alpha 1 (GSTa1) protein
Brand: MetaVital™
Development Stage: ADME
ADME Research Domain: Metabolism
Short Description
Quantitatively measures rat GSTa1 protein levels in biological samples for detoxification and oxidative stress research.
Brand
MetaVital™
Development Stage
ADME
ADME Research Domain
Metabolism
Description
MetaVital™ Rat Glutathione S Transferase Alpha 1 (GSTa1) ELISA Kit provides a sensitive and reliable method for detecting GSTa1 levels in rat serum, plasma, tissue homogenates, and cell lysates. This assay supports studies investigating xenobiotic metabolism, oxidative stress responses, and phase II detoxification mechanisms in pharmacological and toxicological research models.
Features
Highly specific capture and detection antibodies enable selective recognition of rat GSTa1 with minimal cross-reactivity among related glutathione S-transferase isoforms.
Sensitive colorimetric ELISA format allows accurate detection of low-abundance GSTa1 across a wide dynamic concentration range.
Pre-coated microplate and optimized assay reagents streamline experimental workflows and improve consistency across multiple sample batches.
Robust assay design supports efficient high-throughput screening while maintaining strong signal reproducibility and reliable quantitative performance.
Process Relevance
Enables monitoring of GSTa1 expression to evaluate detoxification capacity and oxidative stress responses in experimental rat systems.
Application Stage
Drug discovery, preclinical toxicology, biomarker validation, and metabolic pathway research.
Applications
Quantitative ELISA analysis of GSTa1 in rat serum, plasma, tissue homogenates, and cell lysates to study detoxification pathways, oxidative stress responses, ADME profiling, and xenobiotic metabolism.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Rat Glutathione S Transferase Alpha 1 (GSTa1) protein
Detection Method
Colorimetric
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Assay Measures
Quantitative
Research Areas
Drug metabolism, pharmacokinetics (ADME), toxicology, oxidative stress biology, pharmacology, enzymology, and biomarker discovery, etc.
Size
96 tests
Data Output Type
Quantitative protein concentration measurement using a colorimetric endpoint ELISA assay.
Assay Range
0.31 ng/ml - 20 ng/ml
LOD
0.19 ng/ml
Species
Rat
Assay Measures
Quantitative
Storage
Upon receipt, store the kit in accordance with the storage conditions specified in the product manual.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20°C; storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Glutathione S-transferase Alpha 1 (GSTa1) is a cytosolic detoxification enzyme belonging to the alpha class of GSTs. It catalyzes the conjugation of glutathione to reactive electrophilic compounds, helping protect cells from oxidative damage and xenobiotic toxicity.
Pathways
GSTa1 participates in the glutathione-dependent phase II detoxification pathway. By catalyzing glutathione conjugation reactions, it facilitates detoxification of reactive metabolites generated during xenobiotic metabolism and contributes to cellular redox homeostasis.
For Research Use Only | Not For Clinical Use
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