MHH-ES In Vitro RNA Expression Analysis Assay (Cap Analysis)
CAT#: ITS-1122-YF2190
Target Cell Organism: Human
Target Cell Name: MHH-ES
Assay Type: Gene and Protein Expression Assays
Assay Overview
This assay is to provide MHH-ES-based In Vitro RNA Expression Analysis Assay (Cap Analysis) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
MHH-ES
Target Cell Organism
Human
Target Cell Background
Human Ewings' sarcoma cell line established from the ascites of a 12-year-old Turkish boy with Ewing's sarcoma of the left pelvis (with peritoneal metastasis)
Related Diseases
Ewing Sarcoma
Research Area
Oncology
Assay Name
In Vitro RNA Expression Analysis Assay (Cap Analysis)
Short Description
MHH-ES-cell based In Vitro RNA Expression Analysis Assay (Cap Analysis)
Assay Description
Cap analysis gene expression involves the sequencing of PCR-amplified cDNA tags obtained from the 5' end of mRNA. For the identification of starting sites of transcription, the cap analysis gene expression tech nique has become a useful platform.
Assay Type
Gene and Protein Expression Assays
Assay Type Details
Following transcription, primary transcript RNA is converted into mature RNA by post-transcriptional modifications. Post-transcriptional modification includes three major steps namely the addition of a 5'cap, addition of a 3'polyadenylation tail and RNA splicing. Stability of RNA has been considered as very important for gene expression and proteins at the post-transcriptional level.