ModifiSite™ Acetaldehyde Enzyme Activity Assay Kit

CAT#: ITS-1125-YL13
Product Type: Assay Kit
Species: All Species
Brand: ModifiSite
Development Stage: Research-Grade Protein-Based Assay Development
Short Description
Measures acetaldehyde using a colorimetric method.
Brand
ModifiSite
Development Stage
Research-Grade Protein-Based Assay Development
Description
This ModifiSite™ Acetaldehyde Enzyme Activity Assay Kit provides a sensitive colorimetric method for quantifying acetaldehyde levels in diverse biological matrices. The system utilizes an enzymatic reaction that results in the formation of a colored product directly proportional to the analyte concentration. This reliable tool facilitates research into alcohol metabolism and oxidative stress pathways, offering high sensitivity and reproducibility for studies focused on metabolic regulation and biochemical profiling.
Features
Employs a specific aldehyde dehydrogenase reaction for the colorimetric quantification of acetaldehyde, streamlining metabolic flux studies and ethanol metabolism research in biological samples.
Process Relevance
Monitoring acetaldehyde levels to optimize fermentation yields and evaluate metabolic pathways in vitro.
Application Stage
Ethanol metabolism research and oxidative stress studies.
Applications
Measure acetaldehyde concentrations in biological samples and fermentation broths to study ethanol metabolism and metabolic flux.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565nm
Sample Type
Serum, plasma, urine, food, beverages, biological samples.
Research Areas
Metabolic Research; Toxicology; Biochemistry
Size
100 tests
Data Output Type
Quantitative concentration measurement
Assay Time
30 min
LOD
LOD: 2 µM
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
This reactive intermediate is a primary byproduct of ethanol metabolism and plays a significant role in various biochemical pathways and metabolic disorders. Its monitoring is crucial for understanding alcohol-related metabolic flux and oxidative stress.
Pathways
Acetaldehyde is a central intermediate in the ethanol oxidation pathway, produced by alcohol dehydrogenase (ADH) and further oxidized to acetate by aldehyde dehydrogenase (ALDH). It is involved in the upstream generation of protein adducts and oxidative damage signals.
For Research Use Only | Not For Clinical Use
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