ModifiSite™ Arginase Assay Kit

CAT#: ITS-1125-HM67
Product Type: Assay Kit
Species: All Species
Brand: ModifiSite
Development Stage: Research-Grade Protein & Enzyme Assay Development
Short Description
Measures arginase activity by detecting urea production.
Brand
ModifiSite
Development Stage
Research-Grade Protein & Enzyme Assay Development
Description
The ModifiSite™ Arginase Assay Kit utilizes a urea-based detection method to validate arginase inhibitors for cancer immunotherapy and cardiovascular disease applications. This assay supports research into the regulation of the urea cycle and immune suppression in the tumor microenvironment. By avoiding radioactive isotopes, it provides a safe and efficient means to screen modulators of arginine metabolism.
Features
Urea-based detection avoids radioactive isotopes; Validates arginase inhibitors for cancer immunotherapy and cardiovascular disease.
Process Relevance
Immuno-Oncology (Tumor Microenvironment)
Application Stage
Lead Optimization
Applications
Investigating urea cycle disorders and immune regulation.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 430 nm
Sample Type
Enzyme preparations, serum, plasma, tissue culture samples
Research Areas
Immuno-Oncology; Cardiovascular Disease
Size
100 tests
Data Output Type
Kinetic (Enzyme Activity)
Assay Time
Results in 2 hrs
LOD
1 U/L; 0.3 U/L with a 2 h reaction time
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Arginase hydrolyzes arginine to urea the final step of the urea cycle. Its activity regulates arginine availability for nitric oxide synthesis; upregulation is a mechanism of immune suppression in cancer.
For Research Use Only | Not For Clinical Use
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