ModifiSite™ Diamine Oxidase Assay Kit

CAT#: ITS-1125-YL3
Product Type: Assay Kit
Species: All Species
Brand: ModifiSite
Development Stage: Research-Grade Protein-Based Assay Development
Short Description
Measures DAO activity using a colorimetric method.
Brand
ModifiSite
Development Stage
Research-Grade Protein-Based Assay Development
Description
This ModifiSite™ Diamine Oxidase Assay Kit provides a precise spectrophotometric method for measuring enzyme activity in biological samples through the oxidative deamination of putrescine. The system produces a colored product that correlates directly with the enzyme concentration, offering high sensitivity and minimal interference. This reliable tool facilitates fundamental research into histamine metabolism and intestinal barrier function, supporting comprehensive studies in metabolic regulation and nutrient absorption processes.
Features
Detects DAO activity via a coupled enzymatic system, enabling high-throughput screening of histamine metabolism and intestinal permeability markers in preclinical research environments.
Process Relevance
Monitoring polyamine oxidation to evaluate metabolic flux and enzymatic response in cellular studies.
Application Stage
Enzyme activity screening and metabolic pathway analysis.
Applications
Analyze histamine metabolism and intestinal barrier integrity markers in homogenized tissues and extracellular fluid samples during research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Serum, plasma, tissue homogenates, biological samples.
Research Areas
Gastroenterology Research; Histamine Metabolism; Biochemistry
Size
100 tests
Data Output Type
Quantitative enzyme activity measurement (endpoint)
Assay Time
30 min
LOD
LOD: 0.5 U/L
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
This copper-containing enzyme is primarily responsible for the oxidative deamination of histamine and other biogenic amines in the extracellular space. It plays a crucial role in regulating amine levels and maintaining homeostasis in intestinal and renal tissues.
Pathways
DAO is the primary enzyme in the catabolic pathway of histamine, converting it to imidazole acetaldehyde. It regulates the bioavailability of histamine, thereby modulating downstream signaling through histamine receptors (H1-H4).
For Research Use Only | Not For Clinical Use
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