Short Description
Measures DAO activity using a colorimetric method.
Development Stage
Research-Grade Protein-Based Assay Development
Description
This ModifiSite™ Diamine Oxidase Assay Kit provides a precise spectrophotometric method for measuring enzyme activity in biological samples through the oxidative deamination of putrescine. The system produces a colored product that correlates directly with the enzyme concentration, offering high sensitivity and minimal interference. This reliable tool facilitates fundamental research into histamine metabolism and intestinal barrier function, supporting comprehensive studies in metabolic regulation and nutrient absorption processes.
Features
Detects DAO activity via a coupled enzymatic system, enabling high-throughput screening of histamine metabolism and intestinal permeability markers in preclinical research environments.
Process Relevance
Monitoring polyamine oxidation to evaluate metabolic flux and enzymatic response in cellular studies.
Application Stage
Enzyme activity screening and metabolic pathway analysis.
Applications
Analyze histamine metabolism and intestinal barrier integrity markers in homogenized tissues and extracellular fluid samples during research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Fluorescence Detection (Ex 530 nm / Em 585 nm)
Sample Type
Serum, plasma, tissue homogenates, biological samples.
Research Areas
Gastroenterology Research; Histamine Metabolism; Biochemistry
Datasheet