ModifiSite™ Free Amino Nitrogen Assay Kit

CAT#: ITS-1125-WB17
Product Type: Assay Kit
Species: All Species
Target: Primary amino groups, including α-amino acids, ammonia, and N-terminal amino nitrogens in peptides.
Brand: ModifiSite
Development Stage: Research-Grade Protein-Based Assay Development
Short Description
Quantifies free amino nitrogen (FAN) using a colorimetric (570 nm) method.
Brand
ModifiSite
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ Free Amino Nitrogen Assay Kit provides a simple and accurate solution for quantifying free amino nitrogen in food and beverage samples. Utilizing a stable ninhydrin-based reaction, this high-throughput compatible system enables rapid determination of yeast assimilable nitrogen, supporting essential quality control and fermentation monitoring in brewing and winemaking processes.
Features
Selectivity and Speed: Specifically detects α-amino acids and ammonia using ninhydrin-based chemistry within a rapid 10-minute reaction timeframe.
Exceptional Sensitivity: Achieves reliable quantification across a linear detection range of 0.2 to 10 mM utilizing minimal 5 µL samples.
High-Throughput Compatibility: Features a homogeneous "mix-incubate-measure" format designed for seamless integration into automated laboratory liquid handling systems.
Superior Matrix Stability: Employs stable reagents that ensure accurate nitrogen determination across complex food and beverage matrices without interference.
Process Relevance
Monitoring bioavailable nitrogen levels to ensure yeast health, preventing stuck fermentations and maintaining consistent flavor profiles in brewing.
Application Stage
Utilized in upstream fermentation optimization, raw material quality assessment, and final product shelf-life and stability testing.
Applications
Quantifying yeast assimilable nitrogen in beer, wort, and wine; monitors protein hydrolysis efficiency in food processing; and evaluates metabolic biomarkers in biological research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Primary amino groups, including α-amino acids, ammonia, and N-terminal amino nitrogens in peptides.
Detection Method
Absorbance at 575 nm
Sample Type
Food and beverages
Research Areas
Fermentation technology, food & beverage science, nitrogen metabolism, yeast physiology, agricultural chemistry, etc.
Size
100 tests
Data Output Type
Quantitative concentration of free amino nitrogen measured via colorimetric absorbance at 575 nm.
Assay Time
20 min
Species
All Species
Storage
Store at 4 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Do not freeze. Keep components away from direct sunlight and avoid prolonged exposure to room temperature during use. Ensure containers are tightly sealed after each use to prevent evaporation or contamination. Return all components promptly to 4 ℃ storage after handling.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
Free amino nitrogen (FAN) represents the pool of individual amino acids and small peptides essential for microbial growth. It serves as a critical indicator of nutritional availability and protein degradation in complex biological matrices.
Pathways
FAN is integral to nitrogen assimilation and amino acid biosynthesis pathways in yeast. It regulates upstream proteolytic breakdown of proteins and downstream production of aroma-active volatile compounds.
For Research Use Only | Not For Clinical Use
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