ModifiSite™ Free Amino Nitrogen Assay Kit (CAT#: ITS-1125-WB17)

Quantifies free amino nitrogen (FAN) using a colorimetric (570 nm) method.

ModifiSite

Research-Grade Protein-Based Assay Development

The ModifiSite™ Free Amino Nitrogen Assay Kit provides a simple and accurate solution for quantifying free amino nitrogen in food and beverage samples. Utilizing a stable ninhydrin-based reaction, this high-throughput compatible system enables rapid determination of yeast assimilable nitrogen, supporting essential quality control and fermentation monitoring in brewing and winemaking processes.

Selectivity and Speed: Specifically detects α-amino acids and ammonia using ninhydrin-based chemistry within a rapid 10-minute reaction timeframe.
Exceptional Sensitivity: Achieves reliable quantification across a linear detection range of 0.2 to 10 mM utilizing minimal 5 µL samples.
High-Throughput Compatibility: Features a homogeneous "mix-incubate-measure" format designed for seamless integration into automated laboratory liquid handling systems.
Superior Matrix Stability: Employs stable reagents that ensure accurate nitrogen determination across complex food and beverage matrices without interference.

Monitoring bioavailable nitrogen levels to ensure yeast health, preventing stuck fermentations and maintaining consistent flavor profiles in brewing.

Utilized in upstream fermentation optimization, raw material quality assessment, and final product shelf-life and stability testing.

Quantifying yeast assimilable nitrogen in beer, wort, and wine; monitors protein hydrolysis efficiency in food processing; and evaluates metabolic biomarkers in biological research.

Internal performance validation using reference standards under defined assay conditions.

Primary amino groups, including α-amino acids, ammonia, and N-terminal amino nitrogens in peptides.

Absorbance at 575 nm

Food and beverages

Fermentation technology, food & beverage science, nitrogen metabolism, yeast physiology, agricultural chemistry, etc.