ModifiSite™ G6PDH Inhibitor Screening Kit

CAT#: ITS-1125-WB37
Product Type: Assay Kit
Species: All Species
Target: Quantitative screening and characterization of potential inhibitors for glucose-6-phosphate dehydrogenase activity.
Brand: ModifiSite
Development Stage: Research-Grade Protein-Based Assay Development
Short Description
Screens for G6PDH inhibitors by measuring the rate of NADPH formation using a colorimetric (565 nm) method.
Brand
ModifiSite
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ G6PDH Inhibitor Screening Kit provides a rapid, non-radioactive platform for evaluating glucose-6-phosphate dehydrogenase modulators in drug discovery. This high-throughput compatible system utilizes a colorimetric MTT-coupled reaction to determine inhibition percentages, supporting the development of novel cancer therapeutics and treatments for metabolic disorders with precise, reliable results in just 30 minutes.
Features
Rapid High-Throughput Design: Utilizes a streamlined "mix-incubate-measure" workflow optimized for 96-well and 384-well plates, ensuring efficient screening of extensive chemical libraries.
Unmatched Sensitivity and Range: Employs a highly sensitive colorimetric reagent (565 nm) capable of detecting minute changes in enzymatic inhibition with superior linearity.
Robust Assay Specificity: Formulated with high-purity G6PDH and specialized substrates to minimize background noise and ensure reliable results across diverse sample types.
Flexible Operational Format: Supports both kinetic and endpoint measurement modes, providing versatility for various experimental designs and drug interaction studies.
Process Relevance
Enabling high-throughput screening of chemical libraries to identify potent G6PDH inhibitors for therapeutic development and metabolic modulation studies.
Application Stage
Drug discovery, metabolic flux analysis, and high-throughput lead optimization in pre-clinical research environments.
Applications
Supporting inhibitor characterization, enzyme kinetics studies, and high-throughput screening of synthetic or natural compounds across oncology, immunology, and metabolic research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Quantitative screening and characterization of potential inhibitors for glucose-6-phosphate dehydrogenase activity.
Detection Method
Absorbance at 566 nm
Sample Type
Chemical compounds
Research Areas
Oncology therapeutics, metabolic disorders, hematology, immunology, antioxidant defense mechanisms, etc.
Size
100 tests
Data Output Type
Quantitative enzyme inhibition data obtained through kinetic or endpoint colorimetric signal measurement.
Assay Time
30 min
Species
All Species
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
G6PDH is the rate-limiting enzyme in the pentose phosphate pathway, crucial for producing NADPH and maintaining cellular redox homeostasis. Its dysregulation is heavily implicated in tumor proliferation and oxidative stress response.
Pathways
The enzyme facilitates the conversion of glucose-6-phosphate to 6-phosphogluconolactone, generating NADPH. This pathway provides precursors for nucleotide synthesis and sustains reduced glutathione levels for cellular protection.
For Research Use Only | Not For Clinical Use
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