Short Description
Screens for G6PDH inhibitors by measuring the rate of NADPH formation using a colorimetric (565 nm) method.
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ G6PDH Inhibitor Screening Kit provides a rapid, non-radioactive platform for evaluating glucose-6-phosphate dehydrogenase modulators in drug discovery. This high-throughput compatible system utilizes a colorimetric MTT-coupled reaction to determine inhibition percentages, supporting the development of novel cancer therapeutics and treatments for metabolic disorders with precise, reliable results in just 30 minutes.
Features
Rapid High-Throughput Design: Utilizes a streamlined "mix-incubate-measure" workflow optimized for 96-well and 384-well plates, ensuring efficient screening of extensive chemical libraries.
Unmatched Sensitivity and Range: Employs a highly sensitive colorimetric reagent (565 nm) capable of detecting minute changes in enzymatic inhibition with superior linearity.
Robust Assay Specificity: Formulated with high-purity G6PDH and specialized substrates to minimize background noise and ensure reliable results across diverse sample types.
Flexible Operational Format: Supports both kinetic and endpoint measurement modes, providing versatility for various experimental designs and drug interaction studies.
Process Relevance
Enabling high-throughput screening of chemical libraries to identify potent G6PDH inhibitors for therapeutic development and metabolic modulation studies.
Application Stage
Drug discovery, metabolic flux analysis, and high-throughput lead optimization in pre-clinical research environments.
Applications
Supporting inhibitor characterization, enzyme kinetics studies, and high-throughput screening of synthetic or natural compounds across oncology, immunology, and metabolic research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Quantitative screening and characterization of potential inhibitors for glucose-6-phosphate dehydrogenase activity.
Detection Method
Absorbance at 566 nm
Sample Type
Chemical compounds
Research Areas
Oncology therapeutics, metabolic disorders, hematology, immunology, antioxidant defense mechanisms, etc.
Datasheet