Short Description
Determines beta-glucuronidase activity.
Development Stage
Research-Grade Protein & Enzyme Assay Development
Description
The ModifiSite™ β-Glucuronidase Assay Kit offers phenolphthalein or p-nitrophenol detection for validating prodrug activation and microbial activity. This assay supports the study of drug metabolism, particularly glucuronide conjugation and hydrolysis. It is also used to monitor bacterial contamination and assess the efficacy of glucuronidase-responsive drug delivery systems.
Features
Phenolphthalein or p-nitrophenol detection; Validating prodrug activation and microbial activity.
Process Relevance
Drug Metabolism/Microbiome
Application Stage
ADME / DMPK
Applications
Mucopolysaccharidosis and drug metabolism studies.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Fluorescence Detection (Ex 365 nm / Em 450 nm)
Sample Type
Blood, tissue and bacterial samples
Research Areas
Microbiology; Drug Metabolism
Datasheet