ModifiSite™ β-Glucuronidase Assay Kit

CAT#: ITS-1125-HM139
Product Type: Assay Kit
Species: All Species
Short Description
Determines beta-glucuronidase activity.
Brand
ModifiSite
Development Stage
Research-Grade Protein & Enzyme Assay Development
Description
The ModifiSite™ β-Glucuronidase Assay Kit offers phenolphthalein or p-nitrophenol detection for validating prodrug activation and microbial activity. This assay supports the study of drug metabolism, particularly glucuronide conjugation and hydrolysis. It is also used to monitor bacterial contamination and assess the efficacy of glucuronidase-responsive drug delivery systems.
Features
Phenolphthalein or p-nitrophenol detection; Validating prodrug activation and microbial activity.
Process Relevance
Drug Metabolism/Microbiome
Application Stage
ADME / DMPK
Applications
Mucopolysaccharidosis and drug metabolism studies.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Fluorescence Detection (Ex 365 nm / Em 450 nm)
Sample Type
Blood, tissue and bacterial samples
Research Areas
Microbiology; Drug Metabolism
Size
100 tests
Data Output Type
Kinetic (Enzyme Activity)
Assay Time
Results in 30 min
LOD
10-4 U/L
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Beta-Glucuronidase cleaves glucuronic acid from substrates. Bacterial glucuronidase can reactivate excreted drug-glucuronides causing intestinal toxicity (e.g. with irinotecan).
For Research Use Only | Not For Clinical Use
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