Short Description
Determines glutaminase activity by measuring glutamate.
Development Stage
Research-Grade Protein & Enzyme Assay Development
Description
The ModifiSite™ Glutaminase Assay Kit utilizes a two-step enzymatic reaction to validate GLS1 inhibitors, which block cancer cell metabolism by starving tumors of glutamate. This assay is essential for investigating glutamine addiction in oncology, supporting the development of metabolic therapies. It provides a robust screen for compounds that disrupt the anaplerotic supply of carbon to the TCA cycle.
Features
Two-step enzymatic assay; Validates GLS1 inhibitors for blocking cancer cell metabolism.
Process Relevance
Cancer Metabolism Target ID
Application Stage
Target Validation
Applications
Cancer metabolism research (glutamine addiction).
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Fluorescence Detection (Ex 415 nm / Em 475 nm)
Sample Type
Biological Samples, Purified Enzyme
Research Areas
Oncology (Cancer Metabolism)
Datasheet