ModifiSite™ Glutaminase Assay Kit

CAT#: ITS-1125-HM103
Product Type: Assay Kit
Species: All Species
Brand: ModifiSite
Development Stage: Research-Grade Protein & Enzyme Assay Development
Short Description
Determines glutaminase activity by measuring glutamate.
Brand
ModifiSite
Development Stage
Research-Grade Protein & Enzyme Assay Development
Description
The ModifiSite™ Glutaminase Assay Kit utilizes a two-step enzymatic reaction to validate GLS1 inhibitors, which block cancer cell metabolism by starving tumors of glutamate. This assay is essential for investigating glutamine addiction in oncology, supporting the development of metabolic therapies. It provides a robust screen for compounds that disrupt the anaplerotic supply of carbon to the TCA cycle.
Features
Two-step enzymatic assay; Validates GLS1 inhibitors for blocking cancer cell metabolism.
Process Relevance
Cancer Metabolism Target ID
Application Stage
Target Validation
Applications
Cancer metabolism research (glutamine addiction).
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Fluorescence Detection (Ex 415 nm / Em 475 nm)
Sample Type
Biological Samples, Purified Enzyme
Research Areas
Oncology (Cancer Metabolism)
Size
100 tests
Data Output Type
Kinetic (Enzyme Activity)
Assay Time
Results in 50 min
LOD
0.66 U/L
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Glutaminase converts glutamine to glutamate fueling the TCA cycle in cancer cells. Inhibitors of GLS1 are being developed to target tumor metabolism making this assay key for drug discovery.
For Research Use Only | Not For Clinical Use
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