Short Description
Quantifies GPx activity via NADPH consumption.
Development Stage
Research-Grade Protein & Enzyme Assay Development
Description
The ModifiSite™ Glutathione Peroxidase Assay Kit uses an NADPH-coupled recycling assay to serve as a standard marker for cellular antioxidant capacity against hydroperoxides. This assay validates the function of selenoproteins in protecting cells from oxidative stress. It supports research into selenium deficiency and the role of GPx in cancer and cardiovascular disease.
Features
NADPH-coupled recycling assay; Standard marker for cellular antioxidant capacity against hydroperoxides.
Process Relevance
Mechanistic Toxicology (Redox)
Application Stage
Discovery
Applications
Oxidative stress and selenium deficiency research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 340 nm
Sample Type
Serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Oxidative Stress; Oncology
Datasheet