ModifiSite™ Glutathione Peroxidase Assay Kit

CAT#: ITS-1125-HM126
Product Type: Assay Kit
Species: All Species
Brand: ModifiSite
Development Stage: Research-Grade Protein & Enzyme Assay Development
Short Description
Quantifies GPx activity via NADPH consumption.
Brand
ModifiSite
Development Stage
Research-Grade Protein & Enzyme Assay Development
Description
The ModifiSite™ Glutathione Peroxidase Assay Kit uses an NADPH-coupled recycling assay to serve as a standard marker for cellular antioxidant capacity against hydroperoxides. This assay validates the function of selenoproteins in protecting cells from oxidative stress. It supports research into selenium deficiency and the role of GPx in cancer and cardiovascular disease.
Features
NADPH-coupled recycling assay; Standard marker for cellular antioxidant capacity against hydroperoxides.
Process Relevance
Mechanistic Toxicology (Redox)
Application Stage
Discovery
Applications
Oxidative stress and selenium deficiency research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 340 nm
Sample Type
Serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Oxidative Stress; Oncology
Size
100 tests
Data Output Type
Kinetic (Enzyme Activity)
Assay Time
Results in 20 min
LOD
40 U/L
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Glutathione Peroxidase (GPx) reduces lipid hydroperoxides using glutathione. It is the primary defense against oxidative stress in the cytoplasm relying on selenium as a cofactor.
For Research Use Only | Not For Clinical Use
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