ModifiSite™ Glyoxalase I Assay Kit

CAT#: ITS-1125-HM142
Product Type: Assay Kit
Species: All Species
Brand: ModifiSite
Development Stage: Research-Grade Protein & Enzyme Assay Development
Short Description
Measures Glyoxalase I activity.
Brand
ModifiSite
Development Stage
Research-Grade Protein & Enzyme Assay Development
Description
The ModifiSite™ Glyoxalase I Assay Kit employs a UV kinetic measurement to validate the detoxification of methylglyoxal, relevant in diabetes and aging research. This assay measures the first step in the glyoxalase system, protecting cells from glycative stress. It supports the investigation of advanced glycation end-product (AGE) formation and metabolic defense mechanisms.
Features
UV kinetic measurement; Validates detoxification of methylglyoxal in diabetes and aging research.
Process Relevance
Aging & Diabetes Research
Application Stage
Basic Research
Applications
Methylglyoxal detoxification and diabetes research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 240 nm (Use a UV plate, e.g. 96-well UV plate, cat# P96UV)
Sample Type
Enzyme preparations or biological samples
Research Areas
Diabetes; Aging Research
Size
100 tests
Data Output Type
Kinetic (Enzyme Activity)
Assay Time
Results in 30 min
LOD
4 U/L
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Glyoxalase I detoxifies methylglyoxal a toxic byproduct of glycolysis. It prevents the formation of Advanced Glycation End-products (AGEs) protecting cells from diabetic complications.
For Research Use Only | Not For Clinical Use
Online Inquiry