Short Description
Quantifies hyaluronidase inhibition by measuring the decrease in hyaluronic acid using a turbidimetric (colorimetric) method.
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ Hyaluronidase Inhibitor Screening Assay Kit provides a rapid, turbidimetric solution for identifying hyaluronidase modulators in drug discovery. This high-throughput compatible system measures the degradation of hyaluronic acid at 600 nm, offering a robust "mix-and-read" workflow to evaluate inhibitor potency. It is essential for screening therapeutic candidates targeting cancer metastasis, inflammation, and extracellular matrix remodeling.
Features
High Sensitivity & Speed: Employs a simplified, rapid protocol detecting minute inhibitory changes via turbidimetric measurement of hyaluronic acid degradation.
Robust Assay Format: Optimized for high-throughput 96-well plate compatibility, ensuring reproducible results across diverse chemical libraries and sample types.
Optimized Specificity: Formulated with high-purity reagents to minimize background interference, providing a reliable signal-to-noise ratio for accurate IC50 determination.
Process Relevance
Accelerating drug development by facilitating rapid identification and potency characterization of novel hyaluronidase-modulating compounds in early-stage pipelines.
Application Stage
Lead identification, secondary screening, and quality assessment during the optimization of anti-inflammatory or oncological drug candidates.
Applications
Enabling high-throughput screening of natural products and synthetic libraries, supporting IC₅₀ determination and kinetic inhibition studies across diverse pharmacological research environments.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Hyaluronidase enzyme activity and its inhibition by small molecules or biologics.
Detection Method
Optical Density at 600 nm
Sample Type
Chemical compounds
Research Areas
Oncology therapeutics, dermatology & skin physiology, inflammatory diseases, drug delivery optimization, etc.
Datasheet