ModifiSite™ Hyaluronidase Inhibitor Screening Assay Kit

CAT#: ITS-1125-WB33
Product Type: Assay Kit
Species: All Species
Target: Hyaluronidase enzyme activity and its inhibition by small molecules or biologics.
Brand: ModifiSite
Development Stage: Research-Grade Protein-Based Assay Development
Short Description
Quantifies hyaluronidase inhibition by measuring the decrease in hyaluronic acid using a turbidimetric (colorimetric) method.
Brand
ModifiSite
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ Hyaluronidase Inhibitor Screening Assay Kit provides a rapid, turbidimetric solution for identifying hyaluronidase modulators in drug discovery. This high-throughput compatible system measures the degradation of hyaluronic acid at 600 nm, offering a robust "mix-and-read" workflow to evaluate inhibitor potency. It is essential for screening therapeutic candidates targeting cancer metastasis, inflammation, and extracellular matrix remodeling.
Features
High Sensitivity & Speed: Employs a simplified, rapid protocol detecting minute inhibitory changes via turbidimetric measurement of hyaluronic acid degradation.
Robust Assay Format: Optimized for high-throughput 96-well plate compatibility, ensuring reproducible results across diverse chemical libraries and sample types.
Optimized Specificity: Formulated with high-purity reagents to minimize background interference, providing a reliable signal-to-noise ratio for accurate IC50 determination.
Process Relevance
Accelerating drug development by facilitating rapid identification and potency characterization of novel hyaluronidase-modulating compounds in early-stage pipelines.
Application Stage
Lead identification, secondary screening, and quality assessment during the optimization of anti-inflammatory or oncological drug candidates.
Applications
Enabling high-throughput screening of natural products and synthetic libraries, supporting IC₅₀ determination and kinetic inhibition studies across diverse pharmacological research environments.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Hyaluronidase enzyme activity and its inhibition by small molecules or biologics.
Detection Method
Optical Density at 600 nm
Sample Type
Chemical compounds
Research Areas
Oncology therapeutics, dermatology & skin physiology, inflammatory diseases, drug delivery optimization, etc.
Size
100 tests
Data Output Type
Quantitative kinetic or endpoint measurement of enzyme activity via absorbance spectroscopy (340-600 nm).
Assay Time
30 min
Species
All Species
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
Hyaluronidases degrade hyaluronic acid, a critical glycosaminoglycan maintaining tissue integrity. Pathological overactivity is linked to cancer progression, venom toxicity, and chronic inflammation, making enzyme inhibition a vital therapeutic strategy.
Pathways
The enzyme facilitates ECM remodeling by cleaving HA, influencing CD44-mediated signaling and inflammatory cascades. Downstream, this impacts cell adhesion, migration, and the bioavailability of co-administered drugs.
For Research Use Only | Not For Clinical Use
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