ModifiSite™ MMP-1 Inhibitor Detection Kit

CAT#: ITS-1125-WB32
Product Type: Assay Kit
Species: Human
Target: Interrogates the proteolytic activity and inhibition profiles of the human MMP-1 enzyme.
Brand: ModifiSite
Development Stage: Research-Grade Protein-Based Assay Development
Short Description
Quantifies MMP-1 activity and screens inhibitors using a fluorometric method.
Brand
ModifiSite
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ MMP-1 Inhibitor Detection Kit provides a robust, fluorescence-based platform for high-throughput screening of potential drug candidates. Utilizing a quenched synthetic substrate, this homogeneous "mix-incubate-measure" system offers exceptional sensitivity and a Z' factor> 0.8. Its rapid, automation-ready workflow enables precise characterization of inhibitor potency against MMP-1, a key target in cancer metastasis and rheumatoid arthritis research.
Features
Rapid High-Throughput Format: Employs a simplified "mix-incubate-measure" protocol optimized for 96-well plates to accelerate large-scale compound library screening.
Superior Sensitivity & Precision: Utilizes a highly sensitive thiopeptide substrate to ensure reliable detection of subtle inhibitory effects with minimal background.
Robust Chemical Compatibility: Formulated to maintain enzymatic stability in the presence of various solvents, ensuring consistency across diverse chemical libraries.
Clear Quantitative Readouts: Delivers precise colorimetric data at 412 nm, facilitating the accurate calculation of IC50 values for drug candidates.
Process Relevance
Evaluating inhibitory potency to streamline the identification of lead compounds for extracellular matrix-related therapeutic interventions.
Application Stage
Drug discovery, high-throughput lead optimization, and biochemical characterization of novel inhibitors.
Applications
Assessing candidate potency, characterizing enzyme kinetics, and screening natural extracts for anti-aging or anti-cancer properties across diverse chemical and biological libraries.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Interrogates the proteolytic activity and inhibition profiles of the human MMP-1 enzyme.
Detection Method
Fluorescence Detection (Ex 490 nm / Em 520 nm)
Sample Type
Chemical compounds
Research Areas
Dermatology and skin aging, oncology and metastasis, arthritis and inflammation, tissue engineering, etc.
Size
100 tests
Data Output Type
Quantitative enzyme activity measurements and inhibition kinetics.
Assay Time
75 min
Species
Human
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Matrix metalloproteinase-1 (MMP-1), or collagenase-1, initiates the degradation of interstitial collagens types. This enzymatic activity is fundamental to physiological tissue turnover, while its dysregulation drives pathological states like photoaging and tumor metastasis.
Pathways
MMP-1 operates within the extracellular matrix (ECM) degradation pathway, often triggered by MAPK/ERK signaling or inflammatory cytokines. It acts upstream of gelatinases, facilitating collective cell migration and structural remodeling during wound healing.
For Research Use Only | Not For Clinical Use
Online Inquiry