Short Description
Quantifies MMP-1 activity and screens inhibitors using a fluorometric method.
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ MMP-1 Inhibitor Detection Kit provides a robust, fluorescence-based platform for high-throughput screening of potential drug candidates. Utilizing a quenched synthetic substrate, this homogeneous "mix-incubate-measure" system offers exceptional sensitivity and a Z' factor> 0.8. Its rapid, automation-ready workflow enables precise characterization of inhibitor potency against MMP-1, a key target in cancer metastasis and rheumatoid arthritis research.
Features
Rapid High-Throughput Format: Employs a simplified "mix-incubate-measure" protocol optimized for 96-well plates to accelerate large-scale compound library screening.
Superior Sensitivity & Precision: Utilizes a highly sensitive thiopeptide substrate to ensure reliable detection of subtle inhibitory effects with minimal background.
Robust Chemical Compatibility: Formulated to maintain enzymatic stability in the presence of various solvents, ensuring consistency across diverse chemical libraries.
Clear Quantitative Readouts: Delivers precise colorimetric data at 412 nm, facilitating the accurate calculation of IC50 values for drug candidates.
Process Relevance
Evaluating inhibitory potency to streamline the identification of lead compounds for extracellular matrix-related therapeutic interventions.
Application Stage
Drug discovery, high-throughput lead optimization, and biochemical characterization of novel inhibitors.
Applications
Assessing candidate potency, characterizing enzyme kinetics, and screening natural extracts for anti-aging or anti-cancer properties across diverse chemical and biological libraries.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Interrogates the proteolytic activity and inhibition profiles of the human MMP-1 enzyme.
Detection Method
Fluorescence Detection (Ex 490 nm / Em 520 nm)
Sample Type
Chemical compounds
Research Areas
Dermatology and skin aging, oncology and metastasis, arthritis and inflammation, tissue engineering, etc.
Datasheet