ModifiSite™ MMP-9 Inhibitor Assay Kit

CAT#: ITS-1125-WB25
Product Type: Assay Kit
Species: Human
Target: The catalytic activity of human matrix metalloproteinase-9 (MMP-9/Gelatinase B).
Brand: ModifiSite
Development Stage: Research-Grade Protein-Based Assay Development
Short Description
Quantifies MMP-9 activity and screens inhibitors using a fluorometric method.
Brand
ModifiSite
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ MMP-9 Inhibitor Assay Kit offers a high-performance, fluorescence-based platform for screening potential inhibitors of matrix metalloproteinase-9. This robust, homogeneous system utilizes a quenched synthetic substrate to deliver sensitive and precise kinetic data, facilitating the rapid identification of therapeutic candidates for cancer and inflammatory diseases while streamlining high-throughput drug discovery workflows.
Features
Rapid High-Throughput Detection: Optimized for 96-well formats, this fluorometric assay enables the simultaneous screening of extensive compound libraries with minimal manual intervention.
Superior Analytical Sensitivity: Utilizing a FRET-based peptide substrate, the kit detects subtle inhibitory effects with a wide dynamic range and low background.
Robust Chemical Stability: Components are engineered to withstand common solvent interference, ensuring reliable IC50 determinations across diverse chemical scaffolds and drug candidates.
Streamlined Protocol Integration: The "mix-and-read" workflow eliminates complex wash steps, significantly reducing experimental variability and total processing time for large-scale operations.
Process Relevance
Simplifying the identification of potent MMP-9 antagonists to streamline lead optimization and validation of novel therapeutic candidates.
Application Stage
Lead discovery, preclinical drug screening, and standardized enzymatic potency assessment for drug development pipelines.
Applications
Identifying inhibitors for drug discovery, characterizing enzyme kinetics in diverse biological matrices, and performing comparative potency analysis of novel small molecules and biologics.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
The catalytic activity of human matrix metalloproteinase-9 (MMP-9/Gelatinase B).
Detection Method
Fluorescence Detection (Ex 490 nm / Em 520 nm)
Sample Type
Chemical compounds
Research Areas
Oncology therapeutics, chronic inflammation, cardiovascular pathology, neurodegeneration, wound healing, etc.
Size
100 tests.
Data Output Type
Quantitative enzyme inhibition kinetics and endpoint fluorescence intensity measurement (RFU).
Assay Time
75 min
Species
Human
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
MMP-9 facilitates basement membrane degradation, a prerequisite for leukocyte extravasation and tumor invasion. Quantifying its inhibition is essential for developing therapies targeting pathological tissue remodeling and systemic inflammatory responses.
Pathways
MMP-9 is a downstream effector of the NF-κB and MAPK pathways. It mediates TGF-β activation and processes cytokines, directly influencing epithelial-to-mesenchymal transition and pro-angiogenic signaling cascades.
For Research Use Only | Not For Clinical Use
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