Short Description
Quantifies MMP-9 activity and screens inhibitors using a fluorometric method.
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ MMP-9 Inhibitor Assay Kit offers a high-performance, fluorescence-based platform for screening potential inhibitors of matrix metalloproteinase-9. This robust, homogeneous system utilizes a quenched synthetic substrate to deliver sensitive and precise kinetic data, facilitating the rapid identification of therapeutic candidates for cancer and inflammatory diseases while streamlining high-throughput drug discovery workflows.
Features
Rapid High-Throughput Detection: Optimized for 96-well formats, this fluorometric assay enables the simultaneous screening of extensive compound libraries with minimal manual intervention.
Superior Analytical Sensitivity: Utilizing a FRET-based peptide substrate, the kit detects subtle inhibitory effects with a wide dynamic range and low background.
Robust Chemical Stability: Components are engineered to withstand common solvent interference, ensuring reliable IC50 determinations across diverse chemical scaffolds and drug candidates.
Streamlined Protocol Integration: The "mix-and-read" workflow eliminates complex wash steps, significantly reducing experimental variability and total processing time for large-scale operations.
Process Relevance
Simplifying the identification of potent MMP-9 antagonists to streamline lead optimization and validation of novel therapeutic candidates.
Application Stage
Lead discovery, preclinical drug screening, and standardized enzymatic potency assessment for drug development pipelines.
Applications
Identifying inhibitors for drug discovery, characterizing enzyme kinetics in diverse biological matrices, and performing comparative potency analysis of novel small molecules and biologics.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
The catalytic activity of human matrix metalloproteinase-9 (MMP-9/Gelatinase B).
Detection Method
Fluorescence Detection (Ex 490 nm / Em 520 nm)
Sample Type
Chemical compounds
Research Areas
Oncology therapeutics, chronic inflammation, cardiovascular pathology, neurodegeneration, wound healing, etc.
Datasheet