Short Description
Quantifies monoamine oxidase (MAO) activity and screens inhibitors using a sensitive fluorometric (or colorimetric) method.
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ Monoamine Oxidase Inhibitor Screening Kit provides a sensitive, fluorimetric platform for evaluating MAO-A and MAO-B modulators in drug discovery. This high-throughput compatible system utilizes a homogeneous "mix-incubate-measure" workflow to determine inhibitor potency (IC50) and selectivity. By measuring H₂O₂ production via p-tyramine oxidation, it supports the rapid screening of therapeutic candidates for neurological disorders such as depression and Parkinson's disease.
Features
High Sensitivity & Precision: Employs a fluorometric detection method reaching lower limits of quantification with exceptional signal-to-noise ratios for reliable IC50 determination.
Streamlined Assay Format: Optimized for 96-well or 384-well microplates, enabling rapid execution of high-throughput primary and secondary screening campaigns.
Specific Enzyme Resolution: Features specialized protocols and controls to differentiate between MAO-A and MAO-B isoforms for evaluating compound selectivity.
Robust Reagent Stability: Components are formulated for long-term storage and consistent performance across multiple batches, ensuring reproducible longitudinal research data.
Process Relevance
Facilitating high-throughput lead identification and optimization of small molecules targeting monoaminergic neurotransmitter degradation pathways for neuroprotection.
Application Stage
Lead discovery, hit-to-lead optimization, and preclinical drug candidate profiling for neurological and neuropsychiatric therapeutic development.
Applications
High-throughput inhibitor screening, IC50 profiling, isoform selectivity assays, and characterizing synthetic or natural compound potency in neurological research and drug development workflows.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Human and animal monoamine oxidase A (MAO-A) and monoamine oxidase B (MAO-B) enzymes.
Detection Method
Fluorescence Detection (Ex 530 nm / Em 580 nm)
Sample Type
Chemical compounds
Research Areas
Neuropharmacology, psychiatry, geriatric medicine, medicinal chemistry, oxidative stress, metabolic health, etc.
Datasheet