ModifiSite™ Monoamine Oxidase Inhibitor Screening Kit

CAT#: ITS-1125-WB34
Product Type: Assay Kit
Species: All Species
Target: Human and animal monoamine oxidase A (MAO-A) and monoamine oxidase B (MAO-B) enzymes.
Brand: ModifiSite
Development Stage: Research-Grade Protein-Based Assay Development
Short Description
Quantifies monoamine oxidase (MAO) activity and screens inhibitors using a sensitive fluorometric (or colorimetric) method.
Brand
ModifiSite
Development Stage
Research-Grade Protein-Based Assay Development
Description
The ModifiSite™ Monoamine Oxidase Inhibitor Screening Kit provides a sensitive, fluorimetric platform for evaluating MAO-A and MAO-B modulators in drug discovery. This high-throughput compatible system utilizes a homogeneous "mix-incubate-measure" workflow to determine inhibitor potency (IC50) and selectivity. By measuring H₂O₂ production via p-tyramine oxidation, it supports the rapid screening of therapeutic candidates for neurological disorders such as depression and Parkinson's disease.
Features
High Sensitivity & Precision: Employs a fluorometric detection method reaching lower limits of quantification with exceptional signal-to-noise ratios for reliable IC50 determination.
Streamlined Assay Format: Optimized for 96-well or 384-well microplates, enabling rapid execution of high-throughput primary and secondary screening campaigns.
Specific Enzyme Resolution: Features specialized protocols and controls to differentiate between MAO-A and MAO-B isoforms for evaluating compound selectivity.
Robust Reagent Stability: Components are formulated for long-term storage and consistent performance across multiple batches, ensuring reproducible longitudinal research data.
Process Relevance
Facilitating high-throughput lead identification and optimization of small molecules targeting monoaminergic neurotransmitter degradation pathways for neuroprotection.
Application Stage
Lead discovery, hit-to-lead optimization, and preclinical drug candidate profiling for neurological and neuropsychiatric therapeutic development.
Applications
High-throughput inhibitor screening, IC50 profiling, isoform selectivity assays, and characterizing synthetic or natural compound potency in neurological research and drug development workflows.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Human and animal monoamine oxidase A (MAO-A) and monoamine oxidase B (MAO-B) enzymes.
Detection Method
Fluorescence Detection (Ex 530 nm / Em 580 nm)
Sample Type
Chemical compounds
Research Areas
Neuropharmacology, psychiatry, geriatric medicine, medicinal chemistry, oxidative stress, metabolic health, etc.
Size
100 tests
Data Output Type
Quantitative enzyme inhibition percentage and IC50 values via kinetic or endpoint fluorescence measurement.
Assay Time
35 min
Species
All Species
Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
MAOs catalyze the oxidative deamination of biogenic amines, regulating levels of dopamine, serotonin, and norepinephrine. Dysregulation is linked to neurodegeneration and mood disorders, making enzymatic modulation a vital therapeutic strategy.
Pathways
MAO is localized to the outer mitochondrial membrane, functioning within the monoamine metabolism pathway. It regulates amine neurotransmitter availability and produces hydrogen peroxide as a byproduct, influencing downstream oxidative stress signaling.
For Research Use Only | Not For Clinical Use
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