ModifiSite™ Nitric Oxide Synthase Assay Kit

CAT#: ITS-1125-HM132
Product Type: Assay Kit
Species: All Species
Brand: ModifiSite
Development Stage: Research-Grade Protein & Enzyme Assay Development
Short Description
Determines NOS activity via nitric oxide production.
Brand
ModifiSite
Development Stage
Research-Grade Protein & Enzyme Assay Development
Description
The ModifiSite™ Nitric Oxide Synthase Assay Kit measures nitrite production to investigate endothelial function and inflammatory signaling. This assay validates modulators of NOS activity, supporting research into vasodilation and immune response. It provides a direct readout of nitric oxide synthesis, aiding in the development of cardiovascular and anti-inflammatory drugs.
Features
Measures nitrite production; Investigating endothelial function and inflammatory signaling.
Process Relevance
Endothelial Function Research
Application Stage
Discovery
Applications
Vasodilation and inflammation signaling studies.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 540 nm
Sample Type
Serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Cardiovascular Disease; Inflammation
Size
100 tests
Data Output Type
Kinetic (Enzyme Activity)
Assay Time
Results in 40 min
LOD
0.25 U/L
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Nitric Oxide Synthase (NOS) produces nitric oxide from arginine. The endothelial isoform regulates vascular tone while the inducible isoform produces high levels of NO for immune defense.
For Research Use Only | Not For Clinical Use
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