ModifiSite™ pNPP Phosphatase Assay Kit (CAT#: ITS-1125-HM137)

General phosphatase assay using pNPP substrate.

ModifiSite

Research-Grade Protein & Enzyme Assay Development

The ModifiSite™ pNPP Phosphatase Assay Kit produces a yellow chromogen to serve as a generic screen for phosphatase activity in biological extracts. This assay validates general phosphatase inhibitors and measures the dephosphorylation of substrates. It provides a convenient tool for monitoring enzyme purification and characterizing phosphatase activity in diverse samples.

Yellow chromogen production; Generic screen for phosphatase activity in biological extracts.

General Phosphatase Screening

Hit-to-Lead Screening

General phosphatase inhibitor screening.

Internal performance validation using reference standards under defined assay conditions.

Absorbance at 405 nm

Protein phosphatases

Signal Transduction