ModifiSite™ pNPP Phosphatase Assay Kit

CAT#: ITS-1125-HM137
Product Type: Assay Kit
Species: All Species
Brand: ModifiSite
Development Stage: Research-Grade Protein & Enzyme Assay Development
Short Description
General phosphatase assay using pNPP substrate.
Brand
ModifiSite
Development Stage
Research-Grade Protein & Enzyme Assay Development
Description
The ModifiSite™ pNPP Phosphatase Assay Kit produces a yellow chromogen to serve as a generic screen for phosphatase activity in biological extracts. This assay validates general phosphatase inhibitors and measures the dephosphorylation of substrates. It provides a convenient tool for monitoring enzyme purification and characterizing phosphatase activity in diverse samples.
Features
Yellow chromogen production; Generic screen for phosphatase activity in biological extracts.
Process Relevance
General Phosphatase Screening
Application Stage
Hit-to-Lead Screening
Applications
General phosphatase inhibitor screening.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 405 nm
Sample Type
Protein phosphatases
Research Areas
Signal Transduction
Size
500 tests
Data Output Type
Kinetic (Enzyme Activity)
Assay Time
Results in 30 min
LOD
3 ng
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
p-Nitrophenyl Phosphate (pNPP) is a non-specific substrate hydrolyzed by phosphatases to yield a yellow product. It serves as a generic convenient tool for screening phosphatase activity.
For Research Use Only | Not For Clinical Use
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