ModifiSite™ Sorbitol Dehydrogenase Assay Kit

CAT#: ITS-1125-HM123
Product Type: Assay Kit
Species: All Species
Brand: ModifiSite
Development Stage: Research-Grade Protein & Enzyme Assay Development
Short Description
Quantifies SDH activity via NADH production.
Brand
ModifiSite
Development Stage
Research-Grade Protein & Enzyme Assay Development
Description
The ModifiSite™ Sorbitol Dehydrogenase Assay Kit measures the conversion of sorbitol to fructose, assessing polyol pathway activity in diabetic neuropathy research. This assay validates inhibitors of the sorbitol pathway, which is implicated in tissue damage from hyperglycemia. It supports the study of diabetic complications and fructose metabolism in liver and kidney tissue.
Features
Measures conversion of sorbitol to fructose; Assessing polyol pathway activity in diabetic neuropathy.
Process Relevance
Diabetic Complications Research
Application Stage
Discovery
Applications
Diabetic complications (polyol pathway) research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565 nm
Sample Type
Serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Diabetes (Complications)
Size
100 tests
Data Output Type
Kinetic (Enzyme Activity)
Assay Time
Results in 15 min
LOD
0.1 U/L
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Sorbitol Dehydrogenase converts sorbitol to fructose in the polyol pathway. In diabetes SDH activity is crucial for preventing sorbitol accumulation which causes osmotic damage to nerves and eyes.
For Research Use Only | Not For Clinical Use
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