Development Stage: Research-Grade Protein & Enzyme Assay Development
Short Description
Measures SOD activity by inhibition of superoxide reduction.
Brand
ModifiSite
Development Stage
Research-Grade Protein & Enzyme Assay Development
Description
The ModifiSite™ Superoxide Dismutase Assay Kit utilizes WST-1 reduction inhibition for the high-throughput measurement of SOD activity in oxidative stress panels. This assay validates the first line of antioxidant defense, crucial for cellular survival. It supports the evaluation of SOD mimetics and the assessment of oxidative damage in aging and disease models.
Features
WST-1 reduction inhibition; High-throughput measurement of SOD activity in oxidative stress panels.
Process Relevance
Oxidative Stress Panel
Application Stage
Discovery
Applications
First-line antioxidant defense evaluation.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 440 nm
Sample Type
Serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Oxidative Stress; Neuroscience (ALS)
Size
100 tests
Data Output Type
Kinetic (Inhibition %)
Assay Time
Results in 1 hr
LOD
0.05 U/mL
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Superoxide Dismutase (SOD) dismutates the toxic superoxide radical into oxygen and hydrogen peroxide. It is the first line of antioxidant defense; SOD1 mutations are linked to ALS.