Monkey CD2 Sandwich ELISA Kit-15 pg/mL

This product is an ELISA kit for the in vitro quantitative measurement of monkey T-cell surface antigen CD2 (CD2) concentrations in serum, plasma and other biological fluids.

CD2 detection

CD2

Monkey

Colorimetric

Quantitative

Serum, plasma and other biological fluids

96 tests

Immunoglobulin superfamily

Certificate of quality

Pre-coated 96-well microplate; Standard; Standard diluent buffer; Wash buffer; Detection reagent A; Detection reagent B; Diluent A; Diluent B; TMB substrate; Stop solution; Plate sealer

0.1 ng/mL

0.156 ng/mL-10 ng/mL

1

1) Standard: Prepare the standard with the recommended volume of Standard Diluent Buffer, to make the standard solution. Then use the Standard Diluent buffer to carry out serial dilutions of the standard solution, as instructed in the Protocol.
2) Wash Buffer: Dilute the concentrated Wash Buffer with distilled water, as instructed in the Protocol.
3) Detection Reagent Preparation: Calculate the total volume of working solution required. Dilute Detection Reagent A and Detection Reagent B with Diluent A and Diluent B, respectively, at 1:100.

1) Set standard, test samples and control wells.
2) Aliquot 100 µL of diluted standard into the standard wells.
3) Aliquot 100 µL of Standard Diluent buffer into control (zero) well.
4) Aliquot 100 µL of diluted samples into the sample wells. Incubate for 90 mins at 37 °C.
5) Aliquot 100 µL of Detection Reagent A to each well. Incubate for 1 hr at 37 °C.
6) Wash 3 times.
7) Aliquot 100 µL of Detection Reagent B to each well. Incubate for 30 mins at 37 °C.
8) Wash 5 times.
9) Aliquot 90 µL of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C.
10) Aliquot 50 µL of Stop Solution.
11) Measure the OD at 450 nm.

CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor.

4°C

6 months

For research use only. Intended for use by laboratory professionals.

CD2; CD2 antigen; Erythrocyte receptor; LFA-3 receptor; LFA-2; Lymphocyte-function antigen-2; T11; SRBC; CD2 molecule; Rosette receptor; T-cell surface antigen CD2

CD2; CD2 antigen; Erythrocyte receptor; LFA-3 receptor; LFA-2; Lymphocyte-function antigen-2; T11; SRBC; CD2 molecule; Rosette receptor; T-cell surface antigen CD2

CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor.