Components
Pre-coated 96-well microplate; Standard; Standard diluent buffer; Wash buffer; Detection reagent A; Detection reagent B; Diluent A; Diluent B; TMB substrate; Stop solution; Plate sealer
Detection Range
0.156 ng/mL-10 ng/mL
Reagent Preparation
1) Standard: Prepare the standard with the recommended volume of Standard Diluent Buffer, to make the standard solution. Then use the Standard Diluent buffer to carry out serial dilutions of the standard solution, as instructed in the Protocol.
2) Wash Buffer: Dilute the concentrated Wash Buffer with distilled water, as instructed in the Protocol.
3) Detection Reagent Preparation: Calculate the total volume of working solution required. Dilute Detection Reagent A and Detection Reagent B with Diluent A and Diluent B, respectively, at 1:100.
Assay Procedure
1) Set standard, test samples and control wells.
2) Aliquot 100 µL of diluted standard into the standard wells.
3) Aliquot 100 µL of Standard Diluent buffer into control (zero) well.
4) Aliquot 100 µL of diluted samples into the sample wells. Incubate for 90 mins at 37 °C.
5) Aliquot 100 µL of Detection Reagent A to each well. Incubate for 1 hr at 37 °C.
6) Wash 3 times.
7) Aliquot 100 µL of Detection Reagent B to each well. Incubate for 30 mins at 37 °C.
8) Wash 5 times.
9) Aliquot 90 µL of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C.
10) Aliquot 50 µL of Stop Solution.
11) Measure the OD at 450 nm.
Target/Description
CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor.
Restrictions
For research use only. Intended for use by laboratory professionals.
Datasheet